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20 Jun 2018

Explain the basis of interaction between small nuclear ribonuclear proteins (snRNPs) and the pre-mRNA splicing substrate.

SUBJECT: Cell Molecular Biology

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Beverley Smith
Beverley SmithLv2
21 Jun 2018

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Related questions

A. To make an mRNA transcript from a gene, the RNA polymerase needs to 'know' where to start and to stop. What are the signals and proteins involved in initiating and terminating RNA polymerase activity in E. coli?

B. Alternative splicing is a key feature of the eukaryotic transcription-translation process.

i) In eukaryotes, alternative splicing of pre-mRNAs can result in the production of many structurally distinct proteins from information encoded in a single gene. Explain.

ii) Do lower eukaryotes (such as yeast, Saccharomyces cerevisiae) have the same opportunity to use alternative splicing to generate a vast array of proteins from a single gene? Explain.

iii) What other splicing events can lead to additional diversity in the repertoire of proteins that could be encoded by a single gene? Hint: think of the N and C terminal ends. What about a post-translational mechanism. Name one.

How can a cell remember which gene it needs to express (turn on)and which gene does from one generation to the next?

1.Methylation of cytosine bases

2.heritable chromatin structure

3.both of the above

4. none of the above

If you were to make 2 liters of a 2 molar solution of SodiumChloride (MW 58.4) How many grams of Sodium Chloride would youdissolve?

/1.58.4 /2.29.2 /3.116.8 /4.233.6

After transcription, nuclear RNA is capped, polyadenylated,spliced. After successful splicing, proteins are added to themature mRNA at the exon junction complex and then transported tothe cytoplasm. Which of the following is a NOT a reason why thesemodifications are added to

mRNA?

1.to identify the RNA molecule as mRNA

2.to stabilize mRNA molecule

3.to aid in transport from ribosome to cytoplasm throughpore

4.to signal to the cell that mRNA is ready for translation

A cell is being held in G1 phasedue to DNA damage caused by UV radiation. When the DNA is damaged,p53 is phorphorylated and precents entry into whatphase?

1.M phase 2. s-phase 3.G0 phase 4. none of the above

6. True or false

a_______ Nuclear pore complexes are specialized so that some mediate import and others mediate export

b_______ Membrane-bound and free ribosomes, which are structurally and functionally identical, differ only in the proteins they happen to be making at a particular time.

c_______ Some proteins can be made as either free or membrane-bound proteins. They have the same mRNA but different splicing and poly-adenylation patterns

d_______ a protein that does not have a sorting signal will be tagged for destruction by ubiquitin

e_______ when the hydrophobic signal peptide emerges from the ribosome, it is bound by SRP, which causes a pause in protein synthesis. Synthesis resumes when the ribosome with a bound SRP then binds to the SRP receptor on the cytosolic surface of the rough ER.

f________ A protein cannot leave the ER unless it is correctly folded

g________ All of the glycoproteins and glycolipids in intracellular membranes have their oligosaccharide chains facing the cytosolic side, and all those in the plasma membrane have their oligosaccharide chains facing the outside of the cell.

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