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16 Nov 2018

I understand that differential gene expression occurs in eukaryotes, where every cell contains the entire genome but differentiated cells only express particular genes important for their function. My question is in regards to prokaryotic cells. Do prokaryotic cells exhibit differential gene expression in the way that eukaryotes do? Or do they express all genes in the cell?

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Beverley SmithLv2

18 Nov 2018

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{Reposted Question} Given the scenario/situation, respond to all questions with complete sentences.

Scientists have been studying the effects of Starkagaren Disease, which is regulated by the stgn gene, in cultured normal and breast cancer cell lines. They have fused a yellow fluorescent probe to the end of thestgn gene to study the disease more closely. By adding a probe, it will create a hybrid protein that emits a yellow color under a blue laser. This hybrid protein is known as a fluorescentâtagged protein, meaning that the protein itself will function and fold normally but it will be tagged with a yellow fluorescent probe (YFP) at the end. This allows the scientists to follow the protein through the cell cycle. They find that the stgn protein normally binds to centromeres in normal cells. After looking at cells at various stages of the cell cycle, they find that compared to normal cells, the breast cancer cells that express the Starkagaren Disease display abnormal nuclei, some appear very large, some have tiny nuclei, and some cells do not have a nucleus at all.

a) Because the STGN protein binds to the centromere, the scientists can also study what other structure?

b) After looking the defects of the affected cells, where in the cell cycle would you guess the error occurs? What do you think happens?

c) The stgn gene appears to not be functioning, do you think the scientists are able to see the STGN protein at the centromeres? Why?

d) The scientists want to see what would happen if they could block the stgn gene from being made in normal cells or lung cancer cells, what is one way they could do this?

copperferret659

Given the scenario/situation, respond to all questions with complete sentences.

Scientists have been studying the effects of Starkagaren Disease, which is regulated by the stgn gene, in cultured normal and breast cancer cell lines. They have fused a yellow fluorescent probe to the end of the stgn gene to study the disease more closely. By adding a probe, it will create a hybrid protein that emits a yellow color under a blue laser. This hybrid protein is known as a fluorescentâtagged protein, meaning that the protein itself will function and fold normally but it will be tagged with a yellow fluorescent probe (YFP) at the end. This allows the scientists to follow the protein through the cell cycle. They find that the stgn protein normally binds to centromeres in normal cells. After looking at cells at various stages of the cell cycle, they find that compared to normal cells, the breast cancer cells that express the Starkagaren Disease display abnormal nuclei, some appear very large, some have tiny nuclei, and some cells do not have a nucleus at all.

a) Because the STGN protein binds to the centromere, the scientists can also study what other structure?

b) After looking the defects of the affected cells, where in the cell cycle would you guess the error occurs? What do you think happens?

c) The stgn gene appears to not be functioning, do you think the scientists are able to see the STGN protein at the centromeres? Why?

d) The scientists want to see what would happen if they could block the stgn gene from being made in normal cells or lung cancer cells, what is one way they could do this?

rosecockroach918

Hello can you help me with the following questions:

1. Which part of an udder was transferred to an empty oocyte to clone the sheep Dolly?

a. blastocyst (I think its this one)

b. meiotic spindle

c. intact udder cell

d. nucleus

2. What is the principle of nuclear equivalence?

a. Differentiated cells have lost DNA that codes for other cell types.

b. All nuclei are the same shape.

c. Only nuclei of germ cells contain DNA capable of producing an entire organism.

d. The genome of every somatic cell contains information capable of generating any other cell type in the body.

3. A gene contains 6 exons, numbered 1-6. Which of the following mRNA isoforms is not a possible transcriptional product of this gene?

a. 1-2-3-4-5-6

b. 2-5-3-4

c. 2-4-5-6

d. 5-6

4. Which of the following is a mechanism used to alter gene expression using mRNA transcripts?

a. Different stability (longevity) of mRNA transcripts

b. Inhibition of translation of certain mRNAs

c. Production of small RNA molecules (microRNAs) that block translation

d. All of the above

e. None of the above

5. You have recently started working in an embryology lab and have obtained a large number of unfertilized zebrafish oocytes. You decide to treat the oocytes with RNAse (a protein that will degrade RNAs, but leave DNA proteins intact) prior to fertilizing them. Will the resulting embryos develop into normal fish?

a. Yes, because RNAs from sperm cells can replace those lost from the egg.

b. Yes, because intact DNA is the only factor required for development to proceed.

c. No, because RNAse treatment will damage the structure of the oocyte.

d. No, because maternal mRNAs are required for the early stages of development.

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