Log in
Biology
1
answer
0
watching
417
views
20 Sep 2018

a. Suppose you isolated a mutation in the CRP gene that results in the production of a CRP protein that cannot bind cAMP. What effect would such a mutation have on expression of the lac operon in the presence versus the absence of lactose (assume that glucose is absent in both cases)?

Answer:

b. Suppose you isolated a different mutation in the CRP gene that results in the production of a CRP protein that binds to the CRP-binding site in the lac promoter even when there is no cAMP available. What effect would this mutation have on expression of the lac operon in the presence versus the absence of lactose, if glucose were also available in both cases?

Answer:

Watch
1
answer
0
watching
417
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Reid Wolff
Reid WolffLv2
20 Sep 2018

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

Given the following mutants and conditions, predict the expression level of the of the lac Z gene. Please indicate your answer with one of followings: no expression, basal level of expression, or activated level of expression. Explain why, in the context of activities by activator and repressor:

I. A mutant of E.coli that has a mutation in the repressor gene (Lac I) for regulating lac operon

a. In the presence of glucose, presence of lactose

b. In the presence of glucose, absence of lactose-

c. In the absence of glucose, absence of lactose-

d. In absence of glucose, presence of lactose-

II. A mutant of E.coli that has a mutation in the cAMP binding site of CAP

a. In the presence of glucose, presence of lactose –

b. In the presence of glucose, absence of lactose –

c. In the absence of glucose, absence of lactose –

d. In absence of glucose, presence of lactose -

1) Which of the following mutations would cause transcription from the Lac Operon in the absence of lactose?

A) mutation that removes the DNA site bound by CAP-cAMP mutation of the CAP gene
B) mutation of a gene encoded by the Lac Operon
C) mutation of the Lac Operator
D) A and B

In E.coli the lac operon is controlled by the presence or absence of both lactose and glucose. The lac repressor only allows expression in the presence of lactose, and it is also only activated when glucose levels are low, such that the lac operon is on when lactose is present but glucose is not.

You have cells that are mutant in the gene coding for the Lac repressor (I-). These cells lack the Lac repressor under all conditions. For these mutant cells, state whether the Lac operon will be switched on or off in the following situations, whether the lac repressor (I) is bound to the operator, and whether CAP-cAMP is bound upstream of the promoter.

a. in the presence of glucose and lactose

b. in the presence of glucose and the absence of lactose

c. in the absence of glucose and the absence of lactose

d. in the absence of glucose and the presence of lactose

Related Documents

BIO 3170 Lecture Notes - Lecture 8: Lac Operon, Catabolite Activator Protein, Rooster Teeth
indigomoose336
BIO 311C Chapter Notes - Chapter 18: Start Codon, Tryptophan, Cytosol
ultramarinegoose457
BIO206H5 Lecture Notes - Lecture 1: Operon, Alpha Helix, Recognition Sequence
bluemosquito495

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in