Sanchez, J.E., Gross, P.G., Goetze, R., Walsh, R., Jr., Peeples, W.J. and Wood, Z.A. Evidence of kinetic cooperativity in dimeric ketopantoate reductase from Staphylococcus Aureus. Biochemistry (2015) 54, 21, p3360
Materials & Methods
The Materials & Methods section should be described in sufficient detail to enable others to repeat the experiments. UniProt Accession IDs (http://www.uniprot.org/) should be provided for all proteins that are purified and/or characterized. RCSB IDs (http://www.rcsb.org/pdb/home/home.do) should provided for all proteins relevant to the presented data and have been deposited in the RCSB databank. Names of products and manufacturers should be included only if alternate sources are deemed unsatisfactory. Novel experimental procedures should be described in detail, but published procedures should merely be referred to by literature citation of both the original and any published modifications.
2. A mistake beginner scientist make in describing experimental methods is to provide an unnecessary level of detail such as specifying how many microliters of each reagent was added (e.g. 2 ul of each dNTP was added to the reaction). Why is this a mistake?
3. What information was provided for the reader to allow him/her to repeat the experiment? Did they describe the experiment with enough detail to enable you to repeat it?
4. What, if any, reagents were described with the final concentration in the reaction? What, if any, reagents were described in terms of final quantity? What, if any, reagents were described in terms of actual volumes used. Make sure to indicate what part of the experiment these reagents were used for (e.g. equilibrium Binding Assays)
Sanchez, J.E., Gross, P.G., Goetze, R., Walsh, R., Jr., Peeples, W.J. and Wood, Z.A. Evidence of kinetic cooperativity in dimeric ketopantoate reductase from Staphylococcus Aureus. Biochemistry (2015) 54, 21, p3360
Materials & Methods
The Materials & Methods section should be described in sufficient detail to enable others to repeat the experiments. UniProt Accession IDs (http://www.uniprot.org/) should be provided for all proteins that are purified and/or characterized. RCSB IDs (http://www.rcsb.org/pdb/home/home.do) should provided for all proteins relevant to the presented data and have been deposited in the RCSB databank. Names of products and manufacturers should be included only if alternate sources are deemed unsatisfactory. Novel experimental procedures should be described in detail, but published procedures should merely be referred to by literature citation of both the original and any published modifications.
2. A mistake beginner scientist make in describing experimental methods is to provide an unnecessary level of detail such as specifying how many microliters of each reagent was added (e.g. 2 ul of each dNTP was added to the reaction). Why is this a mistake?
3. What information was provided for the reader to allow him/her to repeat the experiment? Did they describe the experiment with enough detail to enable you to repeat it?
4. What, if any, reagents were described with the final concentration in the reaction? What, if any, reagents were described in terms of final quantity? What, if any, reagents were described in terms of actual volumes used. Make sure to indicate what part of the experiment these reagents were used for (e.g. equilibrium Binding Assays)
