I am trying to understand the assay for iPS cells in theTakahashi & Yamanaka 2006 paper.
They inserted a beta-geo cassette, which contains the neomycinresistance gene, into the Fbx15 gene. The idea is that the beta-geocassette will be expressed when Fbx15 will be expressed, thusconferring G418 resistance to only those cells expressingFbx15.
They say that "Although specifically expressed in mouse ES cellsand early embryos, Fbx15 is dispensable for the maintenance ofpluripotency and mouse development." Does this mean that Fbx15 willbe expressed in induced pluripotent stem cells, and won't beexpressed upon differentiation? (What is special about Fbx15 thatwill distinguish iPS cells from somatic cells, including those thatmight derive from iPS cells, as when pluripotency is notmaintained?)
I am trying to understand the assay for iPS cells in theTakahashi & Yamanaka 2006 paper.
They inserted a beta-geo cassette, which contains the neomycinresistance gene, into the Fbx15 gene. The idea is that the beta-geocassette will be expressed when Fbx15 will be expressed, thusconferring G418 resistance to only those cells expressingFbx15.
They say that "Although specifically expressed in mouse ES cellsand early embryos, Fbx15 is dispensable for the maintenance ofpluripotency and mouse development." Does this mean that Fbx15 willbe expressed in induced pluripotent stem cells, and won't beexpressed upon differentiation? (What is special about Fbx15 thatwill distinguish iPS cells from somatic cells, including those thatmight derive from iPS cells, as when pluripotency is notmaintained?)
