Question

1. What specific genetic manipulation does Synechocystis readilyallow that E. coli does not?

2. For transformation of Synechocystis, why do you first plateyour transformant mixture

on a filter on a BG - 11 plate with glucose, and the next daythe TA transfers the filter

with cells to a plate that contains kanamycin (rather thanplating directly on kanamycin)?

3. What is the product of the psbC gene? What is itsfunction?

4. In the PCRreaction, what is the role of each of the PCR reagents: primers,dNTPs,

Mg2+, and Taq polymerase?

5. What are theconsiderations in determining the number of cycles needed for thePCR

reaction?