Why is it important that the donor cultures are grown to exponential phase, but the recipient cells can be in stationary phase?

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Irving HeathcoteLv2

21 Aug 2019

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What is occurring in the cells as they leave the maximum exponential phase and approaching stationary phase? What could be added or subtracted from the culture that would return the population to its maximum exponential rate?

Hexokinase is the first enzyme in the famous biochemical pathway called glycolysis. There is an imaginary strain of E. coli (strain XYZ) that must be grown in minimal media and maintained in an exponential phase of growth. During exponential growth, rapid cell division occurs and requires the production of large amounts of ATP. Once it reaches the stationary phase where cell division equals cell death, the end-product of glycolysis (pyruvate) builds up in the cytoplasm resulting in cell death due to osmotic imbalance. Propose a theory to explain this observation.

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