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6 May 2019

in a nerve cells botulinum toxin-A degrades a subunit of a T-snare localized on a plasma membrane, how would this affect vesicular trafficking

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Jean KeelingLv2

8 May 2019

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In previous studies it has been observed that exogenously derived amyloid beta and prion protein aggregates co-localize with dextran, a marker for fluid-phase endocytosis (but the beta-amyloid and prion protein aggregates do not co-localize with cholera toxin subunit B, a marker of lipid rafts.

When cells were exposed to MTBR-AF488 Tau aggregates and dextran, it was observed that 24% of Tau aggregates co-localized with Dextran, but they did not co-localize with cholera toxin B. The authors said that this suggests that Tau aggregates enter cells via the endocytotic pathway involving the engulfment by the cell membrane, instead of simple penetration of the membrane

Why is lipid raft marker involved in this study? Why did the authors say that it was an endocytotic pathway instead of simple penetration of the membrane? What's the difference?

brownworm237

Answer the Following A-F

A.What would a cell lacking functional t-SNAREs look like?

B.What are the facilitating roles of SNARE proteins?

C.What is the specific role of NSF and SNAP in vesicle trafficking?

D.During the process of vesicle fusion to target membrane, where is ATP hydrolysis required?

E.What are common features of lysosomal hydrolases?

F.What is the function of cytoplasmic Hsc70 during endocytosis?

Please Help Answer These! I dont understand and my test is tomorrow! Thank You!!!

1) Which of the following statements about transport into the mitochondria is FALSE?

a) The signal sequence on proteins destined for the mitochondria is recognized by a receptor protein in the outer membrane of the mitochondria.

b) Proteins that are transported into the mitochondria are unfolded as they are being transported.

c) Transport of proteins into the mitochondria requires SRP and the SRP receptor.

d) After a protein is made in the cytosol it must move through two protein translocators to reach the inner mitochondrial matrix.

2.Which coat protein is involved in vesicle formation during endocytosis â vesicle forms from plasma membrane and fuses with endosomes?

a) COPI
b) COPII

c) v-SNARES d) Clathrin

3. Which of the following statements about vesicular membrane fusion is TRUE?

a) Membrane fusion does not always immediately follow vesicle docking.

b) The interactions of the v-SNAREs and t-SNAREs pull the vesicle membrane and the target organelle together so that their lipids can intermix.

c) NSF and SNAP disassemble the SNARE complex so the SNAREs can be recycled.

d) All of the above are true.

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in a nerve cells botulinum toxin-A degrades a subunit of a T-snare localized on a plasma membrane, how would this affect vesicular trafficking

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