M4D1 Bioterrorism- which bacterial species would you suspect is the causative agent and why? what features make your selected bacterial species a good candidate for an agent of bioterrorism?

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Casey DurganLv2

19 Jul 2018

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What are the challenges for prevention and control of aparticular infectious disease. Does this disease already have avaccination, and if so, is the challenge in getting people to getvaccinated, or in creating a vaccine, or something else? How doesthe biology of a virus (for example, influenza or HIV) or bacteria(for example, TB or MRSA) present a challenge for its control? Howcan/do some organisms cause pandemics and how are different species(humans, animals) involved in that process? What do you think maybe the next infectious disease pandemic, and why? What organisms doyou think are most at risk for bioterrorism use, and why? Whatefforts are in place to prevent infectious disease relatedbioterrorism, and are they sufficient?

If any references are use can you list them

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Pre-Laboratory Questions

1. Why do bacterial cultures need to be incubated, usually for at least 24 hours, before we can see evidence of bacterial growth that can be observed with the unaided eye?

2. In what ways are the media used in this investigation selective?

3. In what ways are the media used in this investigation differential?

4. Consider a mixture of bacterial species from the same genus, such as Staphylococcus. Some members of this genus are harmful pathogens, while others are not. Would a selective medium alone be a good tool to separate the harmful species from the non-harmful species? Explain your answer.

Post-Lab Questions

1. Would simply adding a pH indicator like phenol red to a general-purpose medium, such as nutrient agar, result in a medium that is selective, differential, both, or neither? Why or why not?

2. Why were you instructed to inoculate each plate with three separate streaks, one of each bacterial species, instead of combining all three species and streaking them all onto the plate together?

3. Blood agar and chocolate agar are commonly-used media in many clinical and research laboratories. Why do you think they were not included in your kit?

4. Were there any discrepancies between what you saw in your experiment and what you expected to see based on the lab manual and your readings in this section?

Explain why a control tube with a nonmotile bacterial species is necessary for reliable determination of bacterial motility by a) the hang drop preparation and b) the motility test agar preparation.