Describe what happens to the lac operon in the presence of both lactose and glucose.

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mints123Lv10

1 Jan 2022

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Jarrod RobelLv2

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In E.coli the lac operon is controlled by the presence or absence of both lactose and glucose. The lac repressor only allows expression in the presence of lactose, and it is also only activated when glucose levels are low, such that the lac operon is on when lactose is present but glucose is not.

You have cells that are mutant in the gene coding for the Lac repressor (I-). These cells lack the Lac repressor under all conditions. For these mutant cells, state whether the Lac operon will be switched on or off in the following situations, whether the lac repressor (I) is bound to the operator, and whether CAP-cAMP is bound upstream of the promoter.

a. in the presence of glucose and lactose

b. in the presence of glucose and the absence of lactose

c. in the absence of glucose and the absence of lactose

d. in the absence of glucose and the presence of lactose

A lac operon of genotype lacI- lacP+ lacO+ lacZ- lacY+ will produce b-galactosidase AND permease when

	lactose is present
	lactose is absent
	in the presence OR absence of lactose
	when glucose is present
	never

2. A lac operon of genotype lacI+ lacP+ lacO+ lacZ+ lacY- will produce b-galactosidase BUT NOT permease when

	lactose is present
	lactose is absent
	in the presence OR absence of lactose
	when glucose is present
	all of the above

goldsquirrel333

1.In the bacterial lac repressor, which of these occurs:

		A.In the presence of glucose and the absence of lactose, the repressor is actively produced.
		B.The lac operon can be hyper-induced in the presence of low glucose by the 'cAMP binding protein' (CAMP).
		C.In the presence of lactose and the absence of glucose the lac operon is repressed.
		D.Allolactose is a byproduct of Î²-galactosidase, indicating high lactose when it builds up.

2 In eukaryotes the enhancer elements can be quite a distance upstream or downstream from the promoter, and transcriptional regulation is helped by protein:protein interactions.

True

False