One strand of a section of DNA isolated from E. coli reads 5â -GTAGCCTACCCATAGG-3â A. Suppose that an mRNA were transcribed using the complement of this DNA strand as a template. What would the sequence of the mRNA in this region be? B. How many different peptides could potentially be made from this sequence of RNA? Would the same peptides be made if the other strand of DNA served as the template for transcription? C. What peptide would be made if translation started exactly at the 5/ end of the mRNA in Part A? When tRNAAla leaves the ribosome, what tRNA will be bound next? When the amino group of alanine forms a peptide bond, what bonds, if any, are broken, and what happens to tRNAAla?
One strand of a section of DNA isolated from E. coli reads 5â -GTAGCCTACCCATAGG-3â A. Suppose that an mRNA were transcribed using the complement of this DNA strand as a template. What would the sequence of the mRNA in this region be? B. How many different peptides could potentially be made from this sequence of RNA? Would the same peptides be made if the other strand of DNA served as the template for transcription? C. What peptide would be made if translation started exactly at the 5/ end of the mRNA in Part A? When tRNAAla leaves the ribosome, what tRNA will be bound next? When the amino group of alanine forms a peptide bond, what bonds, if any, are broken, and what happens to tRNAAla?
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DNA Structure and Function Lab Report
Summary Questions
- Name the three major components of DNA.
- Which nitrogen bases pair within DNA?
- What is the difference between replication, transcription, andtranslation?
- Describe an advantage of genetic engineering.
- List three differences between DNA & RNA.
DNA | RNA |
- If the base sequence on one DNA strand is 3â CCTAGTTAG 5â, whatwill the sequence be on the other strand of the helix duringreplication?
DNA | 3â | C | C | T | A | G | T | T | A | G | 5â |
DNA | 5â | 3â |
- If 3â TGACCCATC 5â serves as the template for transcription,what will be the sequence of nitrogen bases on the newly formed RNAstrand?
DNA | 3â | T | G | A | C | C | C | A | T | C | 5â |
mRNA | 5â | 3â |
- If a mRNA sequence is 3â UAA 5â, give the amino acid coded forduring translation. Refer to the chart in Exercise 5 of theLab.
