Question

Biochemistry

Which of the following pentapeptides is expected to be the BESTdissolved in water?

Select one:

a. NH2 - ser - pro- gly - ala- cys - asn - COOH

b. NH2 - tyr - thr - gly - ser - gln - asp - COOH

c. NH2 - ser - ala - gly - phe - gln - asp - COOH

d. NH2 - gly - leu - phe - val - ser - gly - COOH

A protein has a molecular mass of 200 kDa as determined by gelfiltration (=native molecular weight). When separated by SDS-PAGE(without a reducing agent present) only one polypeptide appearedwith a mass corresponding to 100 kDa. When determined by SDS-PAGEin combination with a reducing agent (DTT) exactly two polypeptidesappeared of sizes 40 and 60 kDa. What is the correct structure ofthe native protein?

Select one:

a. The native protein is made of one 40 kDA and one 60 kDApolypeptides

b. The native protein is made of two 100 kDa polypeptides

c. The native protein is made of two 40 kDA and two 60 kDApolypeptides

d. The native protein is made of four polypeptides - two 100 kDapolypeptides, one 40 kDA and one 60 kDA polypeptides

e. None of the answers is correct.