1.The PCR (polymerase chain reaction) protocol that is currentlyused in laboratories was facilitated by the discovery of abacterium called Thermus aquaticus in a hot spring insideYellowstone National Park, in Wyoming. This organism contains aheat-stable form of DNA polymerase known as Taqpolymerase, which continues to function even after it has beenheated to 95°C.
a.Why would such a heat-stable polymerase be beneficial inPCR?
b.What would happen if it werenâtheat stable?
c.How might you choose a regionof DNA for a PCR primer so as to increase the temperature necessaryfor primer annealing (to minimize nonspecific PCRproducts)?
d.A PCR reaction begins with 5double stranded segment of DNA. Estimate the number ofdouble-stranded copies of DNA that are present after the completionof 15 amplification cycles?
1.The PCR (polymerase chain reaction) protocol that is currentlyused in laboratories was facilitated by the discovery of abacterium called Thermus aquaticus in a hot spring insideYellowstone National Park, in Wyoming. This organism contains aheat-stable form of DNA polymerase known as Taqpolymerase, which continues to function even after it has beenheated to 95°C.
a.Why would such a heat-stable polymerase be beneficial inPCR?
b.What would happen if it werenâtheat stable?
c.How might you choose a regionof DNA for a PCR primer so as to increase the temperature necessaryfor primer annealing (to minimize nonspecific PCRproducts)?
d.A PCR reaction begins with 5double stranded segment of DNA. Estimate the number ofdouble-stranded copies of DNA that are present after the completionof 15 amplification cycles?
