Aspartate transcarbamoylase (ATCase) catalyzes the formation ofN-carbamoyl aspartate from carbamoyl phosphate and aspartate, astep in the multienzyme process that synthesizes cytidinetriphosphate (CTP).
Carbamoyl phosphate + aspartate = N-carbamoylaspartate ===UMP== UTP=CTP
Kinetic studies of ATCaseactivity as a function of aspartate concentration yield the resultsshown in the graph.
NOTE: from graph: WITH ATP;Aspartame (nM) Has a higher % relative activity.
NO CTP OR ATP: theline is between both the above and bottom lines.
with CTP:Aspartame (nM) Has the lowest % relative activity.
a) Is ATCase an allosteric enzyme? How do you know?
b) What kind of an effector is CTP? Explain. What is the biological significance of CTPâseffect on ATCase?
c) What kind of an effort is ATP?Explain. What is the biological significance of ATPâs effect onATCase?
Aspartate transcarbamoylase (ATCase) catalyzes the formation ofN-carbamoyl aspartate from carbamoyl phosphate and aspartate, astep in the multienzyme process that synthesizes cytidinetriphosphate (CTP).
Carbamoyl phosphate + aspartate = N-carbamoylaspartate ===UMP== UTP=CTP
Kinetic studies of ATCaseactivity as a function of aspartate concentration yield the resultsshown in the graph.
NOTE: from graph: WITH ATP;Aspartame (nM) Has a higher % relative activity.
NO CTP OR ATP: theline is between both the above and bottom lines.
with CTP:Aspartame (nM) Has the lowest % relative activity.
a) Is ATCase an allosteric enzyme? How do you know?
b) What kind of an effector is CTP? Explain. What is the biological significance of CTPâseffect on ATCase?
c) What kind of an effort is ATP?Explain. What is the biological significance of ATPâs effect onATCase?
