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a) FLC gene expression is suppressed following vernalization. Describe two epigenetic histone modifications that lead to FLC gene suppression. b) These histone modifications on the FLC gene were originally detected by chromatin immunoprecipitation (ChIP). Using antibodies specific for each of the modified histones (part a) in a ChIP experiment, would the amount of detected FLC DNA increase or decrease during the vernalization period? Explain your answers.
why older women are more likely to have children born with birth defects than younger women?
12. For each of the following, choose one of the listed experimental approaches that you think is the most appropriate to answer the question. Possible approaches: ChIP-PCR, Reverse Transcription-qPCR, Western blot, yeast two hybrid, DNase I footprinting, EMSA, immunohistochemistry (8 pt)
a) Is Gene Z transcribed, ie, is the RNA present, in cell type A?
b) Does transcription factor TF bind to a region within 500 base pairs of Gene Z in vivo in cell type A?
c) Does transcription factor TF bind to RNA polymerase?
d) Is transcription factor TF localized in the nucleus?