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You have discovered a toxin and want to know what it interacts with. You bind your toxin to a solid matrix and use it to perform affinity chromatography with mammalian cell lysate (i.e., broken mammalian cells).
a) What method(s) could you use to determine if any proteins bound to your matrix?
b) What method(s) could you use to quantify the total amount of protein in the starting lysate to the lysate after incubation with your matrix?
c) Assume at least one protein sticks to your toxin-matrix. How could you identify it?
d) Do you think the proteins identified cause toxicity of the toxin? Why or why not?