7) Describe what is meant by the restriction-modificationsystem? be detailed when describing

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Patrina SchowalterLv2

29 Sep 2019

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1)You should understand all the steps that wetook in class to transform Escherichia coli and get thegenes expressed.

2) What role did Restriction Enzymes and DNAligase have in making the PGLO plasmid. What is meant by expressionvectors? What information needs to be in a successful expressionvector?

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1. Hundreds of different restriction endonucleases have been discovered, each with its own recognition sequence. What are the recognition sequences for the enzymes PstI, HindIII, and SspI? New England Biolabs specializes in restriction enzymes and you can look up their recognition sequences in the company catalog online: https://www.neb.com/products.

2. What is meant by a 5â overhang, a 3â overhang, and a blunt end in relationship to restriction enzymes? You may give examples to indicate their meanings.