ASEPTIC TECHNIQUE Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Part I: Contamination Step 6: What color are the trafficlights after caps are removed? Step 7: What color are the trafficlights after caps have been left off and then replaced? Step 9:Appearance of tube: Step 12: Appearance of tube after overnightincubation: Part II: Aseptic Transfer Tube-to-Tube Test Was youraseptic transfer successful? (Contamination light and Inoculationlight both green) Appearance of tube after overnight incubation:Discussion In the Discussion section, answer the followingquestions: What precautions are taken in a "wetlab" to preventcontamination? During the "Contamination" portion of the exercise,why was the contamination not present when you first placed thephenol red glucose tube in the 37 degree incubator? After yoursample had been incubated for one day, why is the solution turbid?What did the color change of the medium indicate? What caused thecontamination of your sample? How successful were you with theaseptic technique? How many times did you need to repeat theexperiment until you were able to successfully inoculate your mediawithout contamination? How important is sterile technique in a"wetlab?" Why? Conclusion < Within a few sentences, provide aconcluding statement about the results of your laboratory>
ASEPTIC TECHNIQUE Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Part I: Contamination Step 6: What color are the trafficlights after caps are removed? Step 7: What color are the trafficlights after caps have been left off and then replaced? Step 9:Appearance of tube: Step 12: Appearance of tube after overnightincubation: Part II: Aseptic Transfer Tube-to-Tube Test Was youraseptic transfer successful? (Contamination light and Inoculationlight both green) Appearance of tube after overnight incubation:Discussion In the Discussion section, answer the followingquestions: What precautions are taken in a "wetlab" to preventcontamination? During the "Contamination" portion of the exercise,why was the contamination not present when you first placed thephenol red glucose tube in the 37 degree incubator? After yoursample had been incubated for one day, why is the solution turbid?What did the color change of the medium indicate? What caused thecontamination of your sample? How successful were you with theaseptic technique? How many times did you need to repeat theexperiment until you were able to successfully inoculate your mediawithout contamination? How important is sterile technique in a"wetlab?" Why? Conclusion < Within a few sentences, provide aconcluding statement about the results of your laboratory>
