Suggest remedies to problems that may occur in the secretory pathway?
Factors in the secretory pathway that may limit the yield and properties of recombinant products
Factor Effect Remedy?
Codon usage/ No transcript/premature ending of transcription
Protein folding / Incomplete or inaccurate folding leading to product degradation
Glycosylation/ Incorrect glycosylation affecting structure, stability, activity and secretion
Proteolytic processing / Incomplete proteolytic cleavage for activity
Proteolytic degradation / Intra-and extracellular host proteases or attack the foreign gene product
Missorting/ Incorrect or incomplete address tags result in mislocalisation and subsequent degradation of a foreign gene product
____________________________________________________
Suggest remedies to problems that may occur in the secretory pathway?
Factors in the secretory pathway that may limit the yield and properties of recombinant products
Factor Effect Remedy?
Codon usage/ No transcript/premature ending of transcription
Protein folding / Incomplete or inaccurate folding leading to product degradation
Glycosylation/ Incorrect glycosylation affecting structure, stability, activity and secretion
Proteolytic processing / Incomplete proteolytic cleavage for activity
Proteolytic degradation / Intra-and extracellular host proteases or attack the foreign gene product
Missorting/ Incorrect or incomplete address tags result in mislocalisation and subsequent degradation of a foreign gene product
____________________________________________________
