Question

Please answer all of the following questions with the best answer.

1.) In Hydrophobic interaction Chromatography, why would we use TE buffer to push out the GFP (green fluorescent protein)?

a.)TE has a low salt concentration, allowing the GFP to refold

b.) the EDTA component of TE inhibits many proteases

c.) the Tris component of TE inactivates the HIC beads

2.) In column chromatography the bed volume is influenced by:

a.) the eluent being applied to the column

b.) the stationary phase

c.) the temperature

d.) the radius of the column

3.) What is the predicted result of increasing amounts of lignin is added to a cellulobiase reaction?

a.) cellulobiase becomes denatured

b.) more product is made

c.) less product is made

d.) Vmax of cellulobiase is decreased

4.) Polypeptides with a hydrophobic core can best be denatured by which of the following treatments?

a.) adding 2M of NaCl to the solution

b.) addition of distilled water

c.) resuspension in TE buffer

d.) multiple freeze-thaw cycles

5.) Intense sound waves can be used to break down bacterial cell walls without damaging he protein of interest.

a.) true

b.) false

6.) Bacteria can be used to make eukaryotic protein.

a.) true

b.) false

7.) pH changes will affect the vicibility of p-nitrophenol.

a.) true

b.) false

8.) A lysozyme pokes holes in a cell wall making it easier for protein to be released from bacteria.

a.) true

b.) false

9.) Bacteria will store excess protein in vesicles.

a.) true

b.) false