Question

-Histone ACETYLATION & DE-ACETYLATION – Histone Acetyl-Transferases (HATs) & Histone De-Acetylases (HDACs) – How do they affect DNA packing? Why?

-Histone Methyl-Transferases (H-MTs) – How do they affect DNA packing? Why? Remember the examples of 1 type of Histone Methylation that is ALWAYS stimulatory and the 1 type of Histone Methylation that is ALWAYS inhibitory.

-What do Nucleosome Remodeling Factors do? What do they need to be active?

-How can miRNAs affect DNA packing? What’s their ultimate effect? What’s the name of the enzyme that mediates that effect (the effect on DNA packaging)?

-DNA-Methyl transferases – What do they do? What’s their effect in DNA packaging?

-Genomic imprinting – What is it? How is it achieved?

-Cis-acting sequences + Core-Promoters + Transcription factors + Co-Activators/Co-Repressors – How do they work together?

-Many transcripts are “frozen” at the early stage of transcription (about 50 nclt away from the +1 position)

-The halting of RNA-Pol II is mediated by a set of 2 factors. What are their names?

-What is the factor that phosphorylated NELF & DSIF? What is the role of that factor?

-What regulates the “recruitment” of that factor onto RNA-Pol II?