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28 Sep 2019
1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain in detail.
2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.
3) In paper electrophoresis, you separated amino acids based on charge. What is the variable this time? How do you know that there is only one variable in this separation?
1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain in detail.
2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.
3) In paper electrophoresis, you separated amino acids based on charge. What is the variable this time? How do you know that there is only one variable in this separation?
Tod ThielLv2
28 Sep 2019