Your laboratory has just gained the license to allow you to research, and commercialize the results for, a novel Protein 77(p77). Because there is very little known about this protein, you need to characterize the protein.
You are provided with:
The cDNA corresponding to the protein, a Chinese Hamster Ovary (CHO) cell line expressing the cDNA for the p77, and the amino acid sequence (using the one-letter code) of the p77.
Shown following is the first 30 amino acid region of this putative amino acid sequence for p77:
1YFNPT 6DSWPT 11LAPAN 16YSFLF 21FLARY 26WYINL30
1. Explain your reasoning in this question for each section based on the partial amino acid sequence provided for p77,
a. which amino acids in the sequence provide may be glycosylated and how can you identify these potential sites? â¨
b. and with which type of glycosylation might they be modified? â¨
c. and explain how these modifications are made, â¨
d. and explain where these modifications are made in the cell,â¨
e. and finally, explain what effects glycosylation might have on the protein.
2. Based on the partial amino acid sequence provided, do you think that it is more likely that this region of p77 associates with a cellular membrane or is exposed to the cytosol?
A) Why or why not?
B) Describe how you would experimentally demonstrate if p77 was associated with the membrane or the cytosol.
Your laboratory has just gained the license to allow you to research, and commercialize the results for, a novel Protein 77(p77). Because there is very little known about this protein, you need to characterize the protein.
You are provided with:
The cDNA corresponding to the protein, a Chinese Hamster Ovary (CHO) cell line expressing the cDNA for the p77, and the amino acid sequence (using the one-letter code) of the p77.
Shown following is the first 30 amino acid region of this putative amino acid sequence for p77:
1YFNPT 6DSWPT 11LAPAN 16YSFLF 21FLARY 26WYINL30
1. Explain your reasoning in this question for each section based on the partial amino acid sequence provided for p77,
a. which amino acids in the sequence provide may be glycosylated and how can you identify these potential sites? â¨
b. and with which type of glycosylation might they be modified? â¨
c. and explain how these modifications are made, â¨
d. and explain where these modifications are made in the cell,â¨
e. and finally, explain what effects glycosylation might have on the protein.
2. Based on the partial amino acid sequence provided, do you think that it is more likely that this region of p77 associates with a cellular membrane or is exposed to the cytosol?
A) Why or why not?
B) Describe how you would experimentally demonstrate if p77 was associated with the membrane or the cytosol.
