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mauverat447Lv1
28 Sep 2019
DNA Replication
1. Assume that mammalian cells are synchronized in the G1 phase of the cell cycle and then cultured in the presence of tritiated thymidine for several hours until they enter mitosis. Will both sister chromatids contain the radioactive label? 2.Will the label be contained in one strand or both strands of DNA in a sister chromatid? 3. If the cells are then allowed to complete two additional cell division cycles in the absence of tritiated thymidine, what will be the distribution of radioactive thymidine within chromosomes at the metaphase stage during each cell division cycle?
DNA Replication
1. Assume that mammalian cells are synchronized in the G1 phase of the cell cycle and then cultured in the presence of tritiated thymidine for several hours until they enter mitosis.
Will both sister chromatids contain the radioactive label?
2.Will the label be contained in one strand or both strands of DNA in a sister chromatid?
3. If the cells are then allowed to complete two additional cell division cycles in the absence of tritiated thymidine, what will be the distribution of radioactive thymidine within chromosomes at the metaphase stage during each cell division cycle?
Irving HeathcoteLv2
28 Sep 2019