Log in
Biology
1
answer
0
watching
451
views

Suppose a mistake is made in preparing a batch of HE agar and the starting pH is 7.0 instead of 7.4-7.8. Would that affect the medium's sensitivity or specificity?

Watch
1
answer
0
watching
451
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Jarrod Robel
Jarrod RobelLv2
28 Sep 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

suppose you make a mistake in preparing a batch of MacConkey agar and the starting pH is 7.6 instead of 6.9-7.3. Would that affect the medium’s sensitivity or specificity? Explain your answer.

Q1. Will the omission of the 7.5% NaCl in MSA medium affect its specificity or sensitivity? Explain.

Q2. Will the addition of only 1/10th of mannitol in the MSA medium affect its specificity or sensitivity? Explain.

Q3. In clinical settings, Blood Agar plates are used to detect b-hemolysin producers. Suppose that after processing a patient’s sample, you saw growth on Blood Agar plate, but with no single colonies. Of the areas that showed growth, you observe full clearing of the blood cells. What conclusions would you draw about the presence of the b-hemolysin producers and a-hemolysin producers? Explain.

Q4. On EMB plates, colonies that would normally appear pink may turn into a metallic green shade if left sitting on the plate for multiple days. Explain why that is the case. Is this a specificity or sensitivity problem?

Q5. One of the features that separate E. coli from closely-related species are its ability to ferment lactose very efficiently. (A) If the lactose in the EMB plate is replaced with glucose, will it affect the specificity or sensitivity of the media?

(B) If the amount of lactose in EMB is reduced by 75%, will that impact the specificity or sensitivity of the medium?

The efficacy of PCR is often assessed in terms of sensitivity, specificity and fidelity.

a) What is the difference between “sensitivity” and “specificity” and “fidelity” of PCR?

b) What PCR conditions affect the sensitivity, specificity and fidelity? List at least 4 in each case separately and briefly explain what happens if each factor is either increased or decreased.

c) How can you experimentally determine the sensitivity, specificity and fidelity of PCR? Describe each case separately

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in