You conduct a trihbybrid cross starting with a parental generation consisting of a female expressing the mutant condition for all three traits and a male that is wild type for all three traits. This cross produces 1000 F2 offspring. The following are phenotypes among the F2 offspring.
Whisker trait spotted belly trait green eye trait # F2 offspring + + + 320 Whiskers Spotted Belly Green eyes 335 + Spotted belly + 161
Whiskers + Green eyes 158 Whiskers Spotted belly + 12 + + Greeny eyes 9 Whiskers + + 3 + Spotted belly Greeny eyes 2
1)Which of the three genes for these traits lies in between the other two?
2) What is the recombination frequency estimated from these data for the whiskers and spotted belly traits?
3) What is the recombination frequency estimated from these data for the whiskers and green eyes traits?
4)What is the chromosome interference value estimated from these data for this region of the chromosome?
You conduct a trihbybrid cross starting with a parental generation consisting of a female expressing the mutant condition for all three traits and a male that is wild type for all three traits. This cross produces 1000 F2 offspring. The following are phenotypes among the F2 offspring.
Whisker trait | spotted belly trait | green eye trait | # F2 offspring |
+ | + | + | 320 |
Whiskers | Spotted Belly | Green eyes | 335 |
+ | Spotted belly | + | 161 |
Whiskers | + | Green eyes | 158 |
Whiskers | Spotted belly | + | 12 |
+ | + | Greeny eyes | 9 |
Whiskers | + | + | 3 |
+ | Spotted belly | Greeny eyes | 2 |
1)Which of the three genes for these traits lies in between the other two?
2) What is the recombination frequency estimated from these data for the whiskers and spotted belly traits?
3) What is the recombination frequency estimated from these data for the whiskers and green eyes traits?
4)What is the chromosome interference value estimated from these data for this region of the chromosome?
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Below are data from a three-point linkage experiment in Drosophila melanogaster. These data represent the offspring from a testcross between flies expressing the recessive mutant phenotypes for black body, vestigial wings and brown eyes and flies that express normal wild type phenotypes but are heterozygous for all three traits. All the heterozygous wild type parental flies in this cross start out with mutant alleles in the cis configuration. Answer the following questions based on these data and show your work for question 1 (a-d).
Body | Wing | Eye | Phenotype | Count |
b+ | vg+ | bw+ | Gray body, normal wings, red eyes | 103 |
b | vg | bw | Black body, vestigial wings, brown eyes | 95 |
b+ | vg | bw | Gray body, vestigial wings, brown eyes | 32 |
b | vg+ | bw+ | Black body, normal wings, red eyes | 29 |
b+ | vg+ | bw | Gray body, normal wings, brown eyes | 71 |
b | vg | bw+ | Black body, vestigial wings, red eyes | 68 |
b+ | vg | bw+ | Gray body, vestigial wings, red eyes | 10 |
b | vg+ | bw | Black body, normal wings, brown eyes | 13 |
1a)Which combinations of phenotypes are only possible in those offspring derived from gametes resulting from double crossover events?
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1b)What is the distance between the body color (gray vs. black) and wing loci (normal vs. vestigial)?
1c)What is the distance between the body color (gray vs. black) and eye color loci (red vs. brown eyes)?
1d)What is the coefficient of coincidence for double crossovers?
The following progeny are obtained from a testcross of a trihybrid wild-type plant to a plant with the recessive phenotypes compound leaves (c), intercalary leaflets (i), and green fruits (g) (Traits not listed are wild types). The testcross progeny are as follows:
Phenotype | Number |
Compound leaves | 324 |
Compound leaves, intercalary leaflets | 32 |
Compound leaves, green fruits | 5 |
Compound leaves, green fruits, intercalary leaflets | 51 |
Intercalary leaflets | 3 |
Green fruits | 42 |
Wild type | 49 |
Intercalary leaflets, green fruits | 309 |
815 |
Determine the order of the three genes, and construct a genetic map that identifies the correct order and the alleles carried on each chromosome in the trihybrid parental plant. Calculate the frequency of the recombination between the adjacent genes in the map. How many double cross-over progeny are expected among the test-cross progeny? Calculate the interference for this cross.