You have just been employed by a small biotech company that, among other things, sequences DNA for individual labs. Your job is to set-up and run the experiments. The first set of reactions you set up fails. All of the reactions stopped within the 1st several bases despite the fact that the reads should have been hundreds of bases long. The individual training you set up some reactions and ran them in parallel to yours and of course all of her reactions were a success. Apparently you have made a rookie mistake and upon inspection of your six reactions it becomes immediately obvious what happened. Here are the data produced from those six reactions. From these data can you determine what your mistake was? How do you know? By the way, each sequence represents what was attached to the primer, which has been omitted from these data (10 points). a. 5Î- ATTGC-3Î
b. 5Î- AC-3Î
c. 5Î-C-3Î
d. 5Î- GGATTATGGC-3Î
e. 5Î- GATC-3Î
f. 5Î- TTAGGAC-3Î
You have just been employed by a small biotech company that, among other things, sequences DNA for individual labs. Your job is to set-up and run the experiments. The first set of reactions you set up fails. All of the reactions stopped within the 1st several bases despite the fact that the reads should have been hundreds of bases long. The individual training you set up some reactions and ran them in parallel to yours and of course all of her reactions were a success. Apparently you have made a rookie mistake and upon inspection of your six reactions it becomes immediately obvious what happened. Here are the data produced from those six reactions. From these data can you determine what your mistake was? How do you know? By the way, each sequence represents what was attached to the primer, which has been omitted from these data (10 points). a. 5Î- ATTGC-3Î
b. 5Î- AC-3Î
c. 5Î-C-3Î
d. 5Î- GGATTATGGC-3Î
e. 5Î- GATC-3Î
f. 5Î- TTAGGAC-3Î
