1. Pre-messenger RNA processing
a. What are the major steps of pre-mRNA processing?
b. How is a cap added to the 5â-end of mRNAs? What is that cap? Can you explain some functions of the cap?
c. What processing happens at the 3â-end of eukaryotic mRNAs? What enzyme is responsible for this? Can you explain some functions of this processing?
d. What is RNA editing? Are you prepared to identify how a single site of editing could change the expression of an mRNA?
e. What are the catalytic sites within an intron? How are they recognized by the spliceosome (you do not have to memorize exact interactions, just understand generally how this occurs)?
f. What is the mechanism of catalysis carried out by the spliceosome? How is this similar to Group I and Group II introns?
g. Are you prepared to answer questions about how splicing defects may result in non-functional protein products?
h. What is alternative splicing and how can it increase the size of the proteome?
1. Pre-messenger RNA processing
a. What are the major steps of pre-mRNA processing?
b. How is a cap added to the 5â-end of mRNAs? What is that cap? Can you explain some functions of the cap?
c. What processing happens at the 3â-end of eukaryotic mRNAs? What enzyme is responsible for this? Can you explain some functions of this processing?
d. What is RNA editing? Are you prepared to identify how a single site of editing could change the expression of an mRNA?
e. What are the catalytic sites within an intron? How are they recognized by the spliceosome (you do not have to memorize exact interactions, just understand generally how this occurs)?
f. What is the mechanism of catalysis carried out by the spliceosome? How is this similar to Group I and Group II introns?
g. Are you prepared to answer questions about how splicing defects may result in non-functional protein products?
h. What is alternative splicing and how can it increase the size of the proteome?
