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Generally, cases of diphyllobothriasis are symptomless, we could think of the situation as the worm lies very still using what it needs and fulfilling its role to reproduce while causing the least amount of trouble for its host. However, what is very often the first sign of worm infestation?

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Casey Durgan
Casey DurganLv2
28 Sep 2019

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Related questions

As you may recall in the work of Triplett (1898), the presence of others in a bicycle race was found to enhance individual performance. This is called social facilitation (Williams, Harkins, & Karau, 2007). At times, however, the presence of others can detract from individual performance, which is termed social inhibition (Crisp & Turner, 2007). Similarly, social loafing can occur when an individual’s performance within a group decreases due to his or her reliance on others in the group (Crisp & Turner, 2007).

For this Discussion, review the media program, Week 5: The Virtual Office. Focus on the characters in the media and determine which characters displayed social facilitation, social inhibition, and social loafing.

With these thoughts in mind:

Post by Day 3 a description of the characters in the media program demonstrating social loafing, social inhibition, and social facilitation. Explain how you identified them. Then, select one of the characters and, using the current literature, explain two ways you might mitigate their behavior.

Media Program

(Onscreen Text: The employees at the United Cardboard Company often work individually on projects, but as with most environments, they are occasionally asked to participate in group projects. One such group is about to meet right now to plan a presentation for the Board of Directors. Observe the conversation and reactions of various group members to determine which group members exhibit signs of the following: Social Loafing, Social Inhibition, and Social Facilitation.)

(Interior of an office. Ken stands at the front of the conference room.)

KEN: OK, I guess we might as well all get started. As we all know, the big man has requested the five of us to get together and write and conceptualize a presentation to the board on our new packaging system. Maybe we can start by going around and throwing out some initial ideas.

KATHY: Sounds good to me. I think a PowerPoint presentation would do the job well.

GAVIN: I agree. We could follow it up with a physical demonstration of how the packaging looks and folds-- maybe a video of the larger pieces that won't fit into the boardroom.

KEN: Sounds perfect to me. Amanda, Brian, what do you guys think?

BRIAN: Yeah, what you guys said sounds fine.

(Amanda thinks, “I don’t know what to say!”)

KEN: Amanda?

AMANDA: I don't know. I didn't really get much chance to think about this. We only found out we needed to do this together three days ago.

KEN: OK, no worries. Let's focus on the PowerPoint idea. I've been doing a lot of them lately, so I'm happy to take the lead on the design aspect. What do you guys think we should all incorporate into the slides?

GAVIN: I think we should start with where we got the idea for this new packaging -- the company that designed it and the different sizes that it comes in.

KATHY: We should follow that with a graph comparing the old prices to the new ones to illustrate how much we'll be saving over time. Amanda, didn't you have most of that information collected at some point?

(Amanda thinks, “Oh no! What if I deleted that?)

AMANDA: I don't know, maybe, it's been so long. I didn't think I'd need it again for a group project like this.

(Gavin thinks, “I wonder what’s gotten into her. She’s not usually this tense.”)

KEN: That's OK, Amanda, I'm sure we can easily dig it up again. Brian, do you have any other ideas for the PowerPoint slides?

BRIAN: Nah, I think what you guys mentioned sounds right.

(Kathy thinks, “Wow. Way to contribute Brian.”)

KEN: OK then, Kathy and Gavin why don't you send me all of the info and I'll write it up and make the slides. Amanda, don't worry about the cost saving document, I'm sure I can dig it up from my old emails and put it into that slide too.

AMANDA: Are you sure? I'm happy to look. I mean I'm sure I have it somewhere. (Kathy thinks, “She really needs to get it together. Why is she so nervous?”) At least I think I have it somewhere. You won't tell the Board of Directors if I lost it, will you?

KEN: Of course not, we're in it together. So as I was saying, I'll write up and prepare all the PowerPoint slides. Then we can start our presentation with reading and explaining each slide. Then, what?

KATHY: Well, and then I think we should show them the packaging live, and the quick deconstructing techniques.

KEN: Great idea. You and Amanda can demonstrate the folding, while I narrate what you're doing.

(Amanda thinks, “Is he doing too much of the work? And what if I drop everything while he’s talking?”)

GAVIN: And after you all demonstrate the smaller packaging, we could show a quick video of how the really big ones function down at the warehouse. Brian, would you be able to go film that quickly one of these days?

BRIAN: I don't really have any experience with video cameras or anything like that, so...

(Gavin thinks, “I should have seen that one coming.”)

KEN: That's OK. My wife actually just got me this great little camera for our anniversary. It takes great videos. So I can just run down to the warehouse, get some footage, then put it together on this new editing software I just downloaded. Maybe add a little music. It's going to be really slick!

KATHY: Are you sure, I wouldn't mind doing that too.

KEN: Oh no, it's fine. I love this kind of stuff; just leave it to me. But I suppose that's enough planning. Now it's time to get to work and make the presentation a knock out. Go team!

(Everyone except Ken thinks, “Team?”) ©2012 Laureate Education, Inc.

2.Conclusion:

What have you learned from your study?

Interpret your data/ results:

Do your results support of disprove your hypothesis?

Compare and contrast your result with those of previous research.

Significance of the results: “Big Picture”

What is the global impact of your results?

What questions remain unanswered?

What kinds of experiments can come out of your study?

i have lab for biology and i need help with the Lab Report

the lab name is Bacterial Transformation Lab

1. Introduction:

Introduce the background and the nature of the problem your experiment is investigating.

Summarize the current knowledge of your subject.

Use examples of other research that have examined the same or a similar topic as your experiments.

Ending your introduction:

State your hypothesis

State your predictions

Give a brief statement about how you tested your hypothesis.

please help me with it is important for my lab class tomorrow

that is the lab i put the info please help me for the intro and for number 2 please be clear please

i would be happy if you help me it is due tommorrow i realy need your help on the intro and number 2 please be clear and show clear work hope you do it because it is due tomorrow

Bacterial Transformation Lab

Genetic engineering is one of the newest applications of basic knowledge in biology. It involves the isolation of specific genes from one organism and the insertion of those genes into a second organism of the same, or even different, species. Remember that a gene is a piece of DNA which codes for a product, usually a protein, which gives an organism a particular trait. The development of these techniques has generated considerable excitement and answered many questions, but it has also raised questions. Many scientists and others see genetic engineering as offering opportunities to make life better, for example, by exploring how genes are regulated, by curing diseases of genetic origin, by adding desirable traits to agricultural crops such as the ability to fix nitrogen in corn and pest resistance in cotton, and by genetically altering bacteria with genes enabling them to digest oil spills.

For almost 100 years, it has been possible to isolate DNA from cells, but only in the last 40 years or so has its function been understood. Your textbook discusses the work of such investigators as Fred Griffith and Oswald Avery, who demonstrated that if DNA was isolated from a pneumonia-causing strain of bacteria and added to cultures of nonvirulent bacteria, the recipient cells were transformed into virulent types that caused pneumonia. These transformation experiments were the first to show that genes could be artificially transferred in the laboratory. Since the time of those experiments (Avery's work was done in the 1940s), our knowledge of gene structure, function and control has increased astronomically. The application of this knowledge has created the field of genetic engineering.

Modern genetic engineering techniques depend on three critical factors:

A suitable host organism in which to insert the foreign gene;

A vector to carry foreign genes into the host; and

A means of isolating the host cells that have taken up the foreign gene.

The host organism

The most commonly used host organism is the gut bacterium Escherichia coli (E. coli). Its chromosome is a single large circular DNA molecule, called the genomic DNA, containing about 4 x 106 base pairs. Not every cell will take up foreign DNA when exposed to it, but E. coli grows rapidly, dividing in less than 20 minutes, so that a single cell can give rise to a billion genetically identical descendants in a matter of 10 hours. Thus, a few transformed cells can provide a large number of offspring within a day. The strain we are using is K-12.

The vector

A vector is a DNA molecule used as a vehicle to carry genes from one organism to another. This DNA molecule can be modified by gene-splicing techniques using restriction enzymes and ligases so that it carries the genes of interest (essentially a cut-and-paste operation). Sometimes viruses are used as vectors, but for E. coli, plasmids are often used. A plasmid is a small circular DNA molecule containing 1000 to 200,000 base pairs. Plasmids exist naturally in many strains of E. coli and other bacteria. Plasmids replicate as the cell grows, independent of replication of the genomic DNA. These plasmids are transmitted to each of the product cells during cell division. Plasmids carrying genetic information that is beneficial to the host cell are maintained in a given population by natural selection. Plasmids frequently carry genes that confer antibiotic resistance, an obvious benefit.

When E. coli cells are exposed to plasmids or any other foreign DNA, some of the cells, called competent cells, will take up the DNA. We can induce cells to become competent by treating them with divalent cations, such as Ca++, followed by a brief exposure to high temperature, called a heat shock treatment. Exactly how this treatment causes cells to become competent is not understood, but it is described as making the membranes "leaky" so that large DNA molecules (like plasmids), which would not normally pass through the cell membrane, can enter the cell. This treatment does not make all cells competent, but it greatly increases the number of competent cells. Once in a cell, the plasmids replicate and are passed to the product cells during cell division.

The selective medium

The last requirement for genetic engineering is a means of isolating host cells that have taken up the gene of interest. The techniques used depend on the growth requirements of the host organism. For example, each species of bacteria has particular nutrient requirements. The composition of the growth medium (agar or broth) can be modified to allow growth of only certain bacteria -- for example, by adding or removing nutrients or other chemicals from the medium mixture. This modified medium is called a selective medium, because it selects for the growth of only some species. By introducing an antibiotic such as ampicillin into the medium in which the bacteria are grown, you will select for growth of only those bacteria carrying the antibiotic-resistance gene on newly acquired plasmids. In a matter of days, billions of antibiotic-resistant cells, all with the plasmid, can be grown.

Genetic engineers use a modification of this technique to insert a foreign gene into E. coli. In simplified terms, the plasmid (already carrying an ampicillin resistance gene) is modified by inserting the gene of interest. The gene of interest has been isolated from another source; for example, the gene for human insulin has been isolated from the human genome. The modified plasmid, carrying the inserted gene, is then mixed with competent E. coli and the bacteria are placed in a medium containing ampicillin. Only those cells that have the plasmid will grow in the medium, and they are also the cells carrying the insulin gene (in their plasmids). These selected cells can now be used to manufacture insulin, which can be used for insulin therapy by diabetics.

The genes

In the following experiment, you will insert a plasmid into E. coli. The plasmid you will use carries the gene bla, which codes for a protein (beta-lactamase) that is secreted by the bacteria, inactivating the ampicillin in the agar and allowing for bacterial growth. The plasmid also carries an indicator gene. The indicator gene we are using was isolated from a bioluminescent jellyfish, Aequorea victoria. It codes for Green Fluorescent Protein (GFP).

The GFP gene has been modified to include a regulator system that can be used to control the expression of the gene. Gene expression in all organisms is carefully regulated to allow for adaptation to differing conditions and to prevent wasteful overproduction of unneeded proteins. The genes involved in the breakdown of different food sources are good examples of highly regulated genes. For example, the sugar arabinose is both an energy source and a carbon source for bacteria. The bacterial genes that make digestive enzymes to break down arabinose for food are not expressed when arabinose is not in the environment. But, when arabinose is present, these genes are turned on. When the arabinose runs out, the genes are turned off.

Arabinose initiates transcription of these genes by promoting the binding of RNA polymerase. In the genetically engineered pGLO DNA, some of the genes involved in the breakdown of arabinose have been replaced by the jellyfish gene GFP. When bacteria with this plasmid are grown in the presence of arabinose, the GFP gene is turned on and the bacteria glow green in UV light. When arabinose is absent form the growth medium, the GFP gene remains turned off and the colonies appear white. This is an example of the central molecular framework of biology in action: DNA ? RNA ? protein ? trait.

In summary, you will render E. coli cells competent to take up plasmid DNA. The plasmid carries two identifiable phenotypic markers:

The beta-lactamase or bla gene codes for resistance to the antibiotic ampicillin. We will use this to select for bacteria that have taken up the plasmid.

The pGLO gene codes for Green Fluorescent Protein (GFP), which is switched “ON” if arabinose is present.

Safety Procedure

For your own safety and the safety of others, it is essential that you follow all instructions about handling bacteria and disposal of materials. When you finish work for the day, swab your table with disinfectant. WASH YOUR HANDS thoroughly with soap before leaving the lab for any reason, and at the end of the day after you have cleaned your table. Report any spills to your instructor.

List of Materials

Prepared agar plates (2 LB, 2 LB/amp, 1 LB/amp/ara)

Transformation solution (50 mM CaCl2, pH 7.4)

LB broth

1 pack of inoculation loops

Sterile transfer pipets

1 foam microtube holder w/ tubes

E. coli starter plate

1 red biohazard bag for loop disposal (common use – Loops/pipets only - NO PAPER!!!)

Read the information on exotix and invasive species and answer this question;

writing assignment : Maximum 1 page answer is needed for the below question

Question:Should the government work to prevent the introduction of exotix species to Canada??

From Freeman et al. 2019. Biological Sciences.

Global trade and global travel have vastly increased the rate of exchange of plants, animals, fungi, and microorganisms around the world. A nonnative species that is introduced into a new area is called an exotic species. Exotic species do not necessarily pose problems, and they can even be beneficial in some circumstances. But some exotic species pose a direct threat to native species by eating them, competing with them, causing disease, or other types of interactions. For example, a lethal fungus is spreading from Asia in the exotic salamander pet trade, endangering native salamanders worldwide. Exotic species can also pose indirect threats by changing the local biotic or abiotic resources.

If an exotic species is introduced to a new area, grows to large population size, and disrupts species native to the area, it is called an invasive species. For example, Burmese pythons were set free by pet owners into the Florida Everglades and are now dramatically affecting native wildlife there. Argentine ants have dispersed around the world in food shipments and are forming supercolonies that outcompete native ants. The round goby, a small benthic fish from Eastern Europe first detected in Ontario rivers in 1990, has since spread to all five Great Lakes. The round goby competes for food and spawning habitat with native fishes and is thought to be responsible for the local extinction of native sculpin species from areas of Lake Michigan.

The impact of invasive species can also be beneficial. McMaster University biologist James Quinn and his colleagues investigated the diet of double-crested cormorant chicks in Lake Ontario to determine whether the parent birds were taking advantage of the newly abundant round goby to feed their young. They found that the round goby was the second most frequent fish species eaten by cormorant chicks, suggesting that piscivorous birds are changing their diet to feed on this invasive fish.

Genetic analysis carried out by Tony Einfeldt, a researcher at the University of New Brunswick, suggests that Hediste diversicolor, an abundant polychaete worm in the Bay of Fundy, is an invasive species that originated in France. H. diversicolor is an important prey item for birds and fish in the mud flats in the Bay of Fundy. The polychaete is a poor swimmer with low dispersal ability and could not have made its own way to Canadian waters. The polychaetes live in mud, so they would not end up in ballast water taken in by ships to balance the mass of their cargo. Einfeldt suggests that these species were likely carried to Canada in the early 1600s by ships used by early European explorers such as Samuel de Champlain and Henry Hudson. Prior to 1880, ships lacked ballast tanks, and sailors loaded ships with intertidal material, including rocks, sticks, and mud, as ‘dry ballast’. Hediste diversicolor likely arrived in the Bay of Fundy in this intertidal mud. The worms form burrows and bring water currents through their burrows as they feed. As a result, H. diversicolor acts as an ecosystem engineer, altering nutrient flow and restructuring the muddy intertidal where it lives. The invasive polychaete is also a major food source for millions of birds and fish in the Bay of Fundy and plays a key role in the ecology of the area. “The reason that many of these birds and fish come to our mud flats is to feed on these prey. There are so many of them,” says Einfeldt. “The idea that so much of what our ecosystem runs on came from our history — it's very exciting.”

Modified from Breining. 2009. A New View on Exotic Species. Yale Environment 360

When biologist Mark Davis talks about invasive species, he eventually comes to LTL, his shorthand for Learn to Love them. Invasive species are here to stay, says Davis, so get used to them, and forget about ripping out the fast-spreading plant purple loosestrife on a large scale or throwing Asian carp on the bank to die. If newcomers are only changing the ecosystem but “not causing significant harm,” then “altering one’s perspective is certainly much less costly than any other sort of management program.”

Davis claims that assuming that invasive species are inherently bad, that ecosystem integrity can be measured by the number of alien species, or even that newly arrived species are functionally different from longtime residents, simply isn’t supported by science. Many introduced species have proven valuable and useful, including crops (from apples to wheat), horticultural plants (hostas and Norway maples), and game species (ring-necked pheasants and brown trout). He holds firm to that position, while conceding that some newcomers can be terribly destructive.

“Some invasive species are causing problems,” admits Davis, who says he supports control programs where damage is great and controls stand a chance of success. One highly destructive invasive species causing great economic harm, for which Davis supports eradication programs, is the emerald ash borer, a beetle—recently introduced into eastern Canada from Asia—that typically kills 99% of ash trees in affected areas.

Today, biologists continue to wage a war on alien species that have taken root by accident or design. Zebra mussels, Dutch elm disease, purple loosestrife, Eurasian water milfoil, American bullfrogs, gypsy moths—Canada is beset by exotic species invading native forests, spreading across prairies, and clogging streams and lakes. Davis takes issue with some conventional views of exotic species.

Because native species evolved in a specific ecosystem and exotics didn’t, natives are better suited to their environment. In fact, says Davis, a scientist who didn’t know the history of individual organisms would have difficulty in distinguishing natives from non-natives. The Galapagos Islands—a world heritage site of biodiversity—provide just such an example. For years, conservationists fretted over invasive weeds overrunning the islands. Because the weeds were out of control, conservationists assumed they were non-natives, recently introduced by humans. Research showed, however, that the plants were native and had been present since prehistoric times.

Diverse communities of native species resist invasions by alien species. While carefully controlled experiments on small plots have confirmed this idea, studies in natural forests and grasslands show the opposite, says Davis. Natural disturbances, such as disease or fire, and fluctuations in resources in diverse communities offer plenty of footholds for invaders.

Pristine ecosystems are highly evolved and well-ordered. Again, ecologists have little evidence for this, Davis says. Ragtag assemblages of exotics and natives quickly adapt and perform about as well. “If you view [nature] as a continually changing tumult, the introduction of new species isn’t necessarily looked at as a huge threat.” He cites the example of regeneration of forests in Puerto Rico, where non-native species are facilitating the re-establishment of native species.

The spread of invasive species threatens to drive natives extinct. Rarely happens, Davis says, except on islands, in lakes, or in other insular environments. Usually it’s the opposite—the appearance of exotics increases species richness, he maintains. Throughout Canada, local ecosystems have roughly 20 percent more plant species than they once did because of the addition for foreign species.

Exotics signify a “degraded” ecosystem. “There isn’t such a thing as a healthy ecosystem or a sick ecosystem,” Davis says. “Ecosystems have no goal or purpose. They’re just the species and the physical and chemical processes taking place.”

In some cases, invasions of invasive species do threaten native species with extinction. Chestnut blight, caused by an introduced fungus, swept across southern Ontario in the 1950s and virtually exterminated the native chestnut tree. Ecologist Dan Simberloff says, “We know it caused total global extinction of at least seven species of moths that were host-specific only on North American chestnuts.”

Even if exotics don’t drive native species to extinction, they can completely transform ecosystems. “There are some biotic communities that have entirely disappeared because of invasive species,” says Simberloff. “There are others that have drastically changed over large areas.” For example, Eurasian cheat grass now dominates millions of hectares of Western range, displacing native bunch grasses and reducing the value of the range for livestock.

James Carlton, Pew Fellow in Marine Conservation, contends that biologists can distinguish natives from exotics. “We can easily distinguish natives from many (not all!) non-natives many times in many ways, based very much on how they function in an ecosystem. Native species are often finely tuned physiologically to the environment, whereas nonnative species may possess a repertoire of adaptations that clearly do not match the environment.”

Conservation biologist Dov Sax became positive about the impact of invasive species as an undergraduate at the University of California, Berkeley. A professor leading a field trip described the Bay Area’s abandoned plantations of Australian eucalyptus trees as a ‘biological desert.’ Says Sax, “There was all kinds of stuff growing in there. I found there were really a similar number of species in both [native oak and eucalyptus] woodland types. Exotics weren’t always doing the awful things people thought they were doing.” Land managers can’t begin to control the thousands of exotics that reach native ecosystems through globalized trade and travel. “Conservation biology in the past has been built around the idea of preventing change,” says Sax, “but it’s impossible to do.”

In a matter of decades, researchers believe, animals and plants are adapting to life in a warmer world. Some species will be unable to change quickly enough and will go extinct, but others will evolve, as natural selection enables them to carry on in an altered environment. With global warming pushing some animals and plants to the brink of extinction, conservation biologists are now saying that the only way to save some species may be to move them. The future landscape will be home to “novel ecosystems,” never-before-seen agglomerations of species, of which exotics will be a key — and often valued component, Sax and others say. “If we lump them into this category of all being evil or awful in some way,” he says, “we may blind ourselves to those situations where they’re actually providing a benefit either for humans or for biological conservation.” For example, a recent study of two nonnative wetland plants, suggests they provide waterfowl habitat, biomass production, and nitrogen retention that equals that of native species. Davis feels we should focus instead on disease organisms, agricultural pests, and other threats and says, “It’s important to distinguish harm from change.”

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