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What happens when the vector dna is digested with EcoRI? What does this tell you about the number and distribution of EcoRI sites in the vector?

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Keith Leannon
Keith LeannonLv2
28 Sep 2019

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Related questions

You have been asked to clone the first enzyme of the leucine biosynthesis pathway from E. coli. To accomplish this objective, you have decided to purify the genomic DNA and treat with EcoRI (5’-G?AATTC-3’) restriction endonuclease. The genome size for E. coli is 6x106 bp (base pairs). After treatment with EcoRI with proper reaction conditions, you have found that the DNA was not digested by the restriction endonuclease EcoR1. However, when the same purified genomic DNA was treated with another restriction endonuclease BalI (5’-TGG?CCA-3’), the DNA was completely digested. The commercially purchased control E. coli DNA showed complete digestion under similar reaction conditions confirming that the EcoRI enzyme is functional and that the E. coli chromosome has multiple EcoRI restriction sites. What is the most probable cause for the E. coli DNA was not be digested by the EcoRI enzyme?

We have three plasmids: the cloning vector, pGEM, the one thatresult if BamHI had been used to construct the genomic library, andthe one that would result if EcoRI had been used to construct thelibrary. What is the expected number of DNA fragments that wouldresult from digesting...

pGEM with the restriction enzymes BamHI, EcoRI, and BamHI +EcoRI?

pGEM + BamHI insert with the restriction enzymesBamHI, EcoRI,and BamHI + EcoRI?

pGEM + EcoRI insert with the restriction enzymesBamHI, EcoRI,and BamHI + EcoRI?


1. A restriction enzyme has a 6 base recognition sequence. How often would it cut DNA in a typical genome?

2. A linear DNA fragment is cut with EcoRI and HindIII:

EcoRI: 1,5,6 kb fragments

BamHI: 3,9 kb fragments

EcoRI and BamHI together: 1,2,4,5 kb fragments

What is the arrangement of the restriction enzyme sites (E=EcoRI, B=BamHI, numbers indicate distance in kb between restriction enzyme sites)?

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