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Based on Bonnie Bassler’s ted talk “How bacteria talk”

Why was it useful for scientists to work with bacteria that generated light?

How might understanding bacteria better result in new medications to combat bacteria?

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Sixta Kovacek
Sixta KovacekLv2
28 Sep 2019

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Related questions

Scientific Method In Action
The Strange Case of BeriBeri
In 1887 a strange nerve disease attacked the people in the Dutch East Indies. The disease was beriberi. Symptoms of the disease included weakness and loss of appetite, victims often died of heart failure. Scientists thought the disease might be caused by bacteria. They injected chickens with bacteria from the blood of patients with beriberi. The injected chickens became sick. However, so did a group of chickens that were not injected with bacteria.
One of the scientists, Dr. Eijkman, noticed something. Before the experiment, all the chickens had eaten whole-grain rice, but during the experiment, the chickens were fed polished rice. Dr. Eijkman researched this interesting case and found that polished rice lacked thiamine, a vitamin necessary for good health.
1. State the Problem
2. What was the hypothesis?
3. How was the hypothesis tested?
4. Should the hypothesis be supported or rejected based on the experiment?
5. What should be the new hypothesis and how would you test it?

4. The table is from work done by Oswald Avery’s research group regarding transformation. The “transforming principle preparation” column on the left refers to the heat-treated smooth (virulent) bacteria. SIII refers to smooth bacteria. R refers to rough (nonvirulent). (this table can be search on google by typing the question. Please search to view it because I can't be able to post it. Thanks)

a. Which of the following best describes transformation?

A. The uptake of foreign material into a cell.

B. The conversion of a non-disease-causing bacteria into a disease causing one.

C. The transfer of novel DNA into a cell.

b. Which of the following best describes how Avery contributed to our understanding that DNA is the genetic material based on his transformation experiments?

A. He showed that non-disease causing bacteria could become disease causing bacteria.

B. He showed that addition of a nuclease to a cell sample could block transformation.

C. He showed that addition of a ribonuclease to a cell sample could block transformation.

D. He showed that addition of a protease to a cell sample could block transformation.

c. Which of the following conclusions would you make based on the table above? Select all that apply. A. DNA, and not RNA or protein, is our genetic information.

B. More “transforming principle preparation” results in more transformation.

C. All replicates of the experiment produced identical results.

D. Without “transforming principle preparation” added to the reaction, transformation cannot happen.

BACTERIAL GROWTH AND CONTROLLING BACTERIALGROWTH

1. Based upon your results, is your bacteria sample a strictaerobe, strict anaerobe, facultative anaerobe, or microaerophile?Why? – Look at your results from OF Glucose and OF Glucose with oiltest.

2. Did the bacteria grow in the tryptone or malonate media? Whyor why not? – Remember, you can tell if they grew if culture wasturbid (cloudy).

3. For the media used in this portion of the experiment, are themedia complex or synthetic? Are the media selective, differential,both, or neither? Identify the carbon and nitrogen source for eachmedium. – Read more about what these terms mean starting on page178 in your book right below Figure 6.11. Look up each media withthe M? button. Remember, nutrient broth is a complex media, mineralsalts is a defined media.

4. How many possible bacteria were remaining after conductingthese biochemical tests?

5. Using the information in the Identification matrix, whatadditional tests might help you to identify the bacteria in yourcase study? –Look at the View Identification Matrix under the Viewmenu. What tests would you perform? Look for ones that have a mixof + and – because they would help distinguish betweenorganisms.

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