Question

You wanted to know whether the expression of a specific gene in different areas of the brain could enhance memory. You are working with fruit flies and have devised a simple memory test that can be conducted with ease. Describe how you would achieve specific expression using the GAL 4 UAS system and why this would be a powerful approach. In addition describe how you would use in situ hybridization to confirm that you were expressing the gene where you thought you were. Finally describe how the second part would be done differently if you used GFP as a reporter gene instead on in situ hybridization.