Question

A group of enzymologists wanted to find a form of alkaline phosphatase capable of maintaining high specific activity at high temperatures. First, they purified alkaline phosphatase from bacteria that live in hot springs. Next, they studied the effects of pH and temperature on specific activity. Finally, because alkaline phosphatase is a metalloprotein, they evaluated the effects of different metal ions on specific activity. 1.What reaction does alkaline phosphatase catalyze? Identify the substrate (type of molecule), any other reactants and the product(s). 2.What organisms make alkaline phosphatase enzymes? 3.Define specific activity and indicate units. Be sure you distinguish between