Question

The DNA duplexes below are denatured and then allowed toreanneal. (a) Which of the two molecules would have the highest Tm?(b) Which of the two is least likely to re-form the originalstructure? Why?

(#1) 5′-ATA TCA TATG ATA TGT A-3′ 3′-TAT AGT ATA CTAT ACAT-5′

(#2) 5′-CGG TAC TCG TGC AGG T-3′ 3′-GCC ATG AGC ACG TCC A-5′