Question

You study a transmembrane protein that is trafficked through theendoplasmic reticulum but ultimately “lives” in the plasmamembrane. Suppose the domain of this protein which is exposed onthe outer face of the endoplasmic reticulum membrane was covalently(and specifically) labeled with a fluorescent dye in a living cell.When the protein is distributed among cellular compartments, wherewould you expect the dye to be located?

Question 7 options:

A) The dye will be on the outer face of the endoplasmicreticulum, on the inner face of the Golgi apparatus, and on theextracellular face of the plasma membrane.

B) The dye will be on the inner face of the endoplasmicreticulum, on the inner face of the Golgi apparatus, and on theintracellular face of the plasma membrane.

C) The dye will be on the outer face of the endoplasmicreticulum, on the outer face of the Golgi apparatus, and on theintracellular face of the plasma membrane.

D) The dye will be on the outer face of the endoplasmicreticulum, on the outer face of the Golgi apparatus, and on theextracellular face of the plasma membrane.

Eukaryotic cells manufacture fibrous cytoskeletal proteins thatfunction in the cytoplasm to maintain cell shape by providingstructural support, move materials within the cell, and move theentire structure. Which of the following predictions about proteinsynthesis, processing, and trafficking applies to the general classof cytoskeletal proteins?

Question 8 options:

A)	They would be glycosylated in the Golgi apparatus.

B)	They might travel to the nucleus in a transport vesicle with aspecific zip code.

C)	They would initially contain signal sequences that would allowtheir entrance into the endoplasmic reticulum.

D)	They would be manufactured on free ribosomes in thecytoplasm.