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What are restriction enzymes, where are the isolated from (and how does this relate to the name of othe restriction enzyme?) what do they do, and how are they used in making recombinant organisms?

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Collen Von
Collen VonLv2
28 Sep 2019
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Related questions

DNA is isolated from person X and is seperately digested with restriction enzymes EcoRI and Hhal. What is the expected occurrence of restriction sites for Hhal? Which enzyme is expected to produce the lower % of smaller restriction fragments? Which enzyme is expected to have restriction sites at the lower frequency in the human genome?

1) Multiple answer. Restriction endonucleases have a number ofcharacteristics, including:

a) They were primarily isolated from phage DNA, such asbacteriophage lambda.

b) They can cut palindromic DNA sequences.

c) They are used to digest a PCR product and a plasmid and withthe aid of ligase, can be used to generate recombinant DNA such asfor protein expression.

d) They hydrolyze recombinant DNA at the ribose base.

e) They have been primarily isolated from bacteria.

1.Whati is Genetic Technology?What is bacterial transformation?
What is recombinant DNA technology?What is a vector?What is found in a typical plasmid?How can DNA sequenced use the dideoxy chain termination method? Describe the polymerase chain reaction (PCR). What are some advantages and limitations of this procedure?What is a cDNA? How are cDNAs and DNA microarrays used to study gene expression?Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology,Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA moleculOutline the procedures for cloning a eukaryotic gene in a bacterial plasmid.Describe techniques that allow identification of recombinant cells that have taken up a gene of interest.How can you use the lacZ gene to facilitate cloning ?Distinguish between genomic and cDNA libraries.Define genomics and proteomics.Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene.How does dideoxy sequencing work? What is the significance of using dideoxy nucleotides?What are BACs, YACs, and contigs? What are some practical applications of DNA technology?What are the ways in which organisms can be genetically modified?

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