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Imagine a red blood cell containing 300 mOsm NaCl is placed in a solution containing 100 mOsM NaCl and 200 mOsM urea. The solution is therefore (hyposmotic, hyperosmotic, or isomotic) and (hyposmotic, hyperosmotic, or isosmotic) to the cell and therefore the cell volume would (decrease, increase, or stay the same size). Please choose the three correct answers from the parentheses and explain.

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Bunny Greenfelder
Bunny GreenfelderLv2
28 Sep 2019
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Related questions

16) Red blood cells with an internal osmolarity of 300 mOsM are placed in the following solutions. Designate each solution according to its osmolarity and tonicity, and explain what happens to the cells and why.

A. 200 mOsM NaCl

B. 400 mOsM urea

C. 100 mOsM urea plus 200 mOsM NaCl

D. 300 mOsM urea

E. 300 mOsM NaCl

F. 200 mOsM urea plus 300 mOsM NaCl

G. 400 mOsM NaCl

Please solve separately and explain thoroughly . Thank you

1. Your goal is to make an isotonic intravenous solution for an emergency room patient who

has lost a lot of blood. The available solutes include glucose (300 mM), NaCl (150 mM), and
urea (300 mM). What solutes should she use to make up the IV solution with an osmolarity
of 300 mOsm (isosmotic), making sure that it will also be isotonic?

Explain your answer.
Assume that the cell is permeable to urea, glucose, but not to NaCl and that the osmolarity of
the cell is 300 mOsm.

2. If a 10% sucrose solution is separated from a 20% sucrose solution by a membrane
impermeable to sucrose, in which direction will net movement of water occur?
A) from the 10% sucrose solution to the 20% sucrose solution only
B) from the 20% sucrose solution to the 10% sucrose solution only
C) There will be no net movement of water in this case.
D) None of the answers are correct.

Please answer all the questions answering only one question is not helpful :

Calculations on Multiple Stock Solutions—be sure to include the amount of water added and include all units

11. Calculate how to use the 1 M Tris and 0.2 M EDTA stock solutions to make the 400 ml of a of 10 mM Tris and 1 mM EDTA solution.

12. Calculate how to use a 3 M NaCl and 10% NP40 stock solutions to make a 500 ml of a 300 mM NaCl and a 0.2% NP40 working solution.

Calculations on Simple Dilutions—be sure to include the amount of water added and include all units

13. Calculate the volumes necessary to make 200 ml of a 1:5 dilution of a sample.

14. Calculate the volumes necessary to make 50 ml of a 1:8 dilution of a sample

15. Calculate how you would dilute a cell suspension containing 3,000,000 cells/ml to obtain 10 ml with 100,000 cells/ml

16. Calculate the volume of a cell suspension containing 800,000 cells/ml you would need to obtain a total of 250,000 cells

Practice Calculations on Serial Dilutions—be sure to include the amount of water added and include all units

17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.

18. Calculate and describe (or diagram) how you would use a 5 M Tris solution to prepare 200 ml of each of the following solutions: 1M, 100 mM ,10 mM , 1 mM, 0.1 mM.

How much of the first dilution should you make to perform the following dilutions?

19. 300 ml of a 1:5 dilution

20. 100 ml of a 1:10 dilution

21. What is the minimum volume you should pipette with the following? (include units with your answers):

P2 P10 P20 P100 P200 P1000

22. How accurate was your pipetting?

23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.

(If there was a consistent problem with one pipettor please tell the instructor.)

Standard Curves

24. Complete a standard curve for the data above and tape a printed version into your notebook.

25. What is the R-squared value of your trend line? Is this good or bad… i.e. would you repeat the assay?

26. What is the protein concentration of (Explain/show your calculations.)

Unknown #1 = Unknown #2 =

Data for DNA Concentration Procedure

Attach a labeled printout of the plate reader raw data as well as your generated standard curve to this lab.

26. What is the concentration of your unknown DNA sample?

27. What is the R 2 for your standard curve data? Why is it important that the R2 is >0.95?

28. Comment on the purity of your DNA... discuss- How can you determine the purity of DNA? What are common contaminants of DNA purified from cells?

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