Microbiology Homework Help !!!
Dan was feeling terrible! He knew he should have worn more sunscreen on that trip to Haiti. Sitting on the plane waiting to be allowed off was torture! He was burning up and felt exhausted. And sitting still so long on the plane had made his ankles and shoulders start to ache too. If only they would let him off the plane! He was beginning to feel nauseous and hoped he didnât vomit all over his fellow passengers.
After they finally let him off the plane he rushed to the bathroom to put some cool water on his face and inspect this horrible sunburn. But it didnât look normal in the mirrorâ¦. His skin was covered in red blotchy spots, especially his chest and all the way up his neck. Maybe he was allergic to something? Can allergies cause this heat (he assumed now it was a fever)? He had to go to the doctor, now. LA General was a short cab ride away.
As the PA on duty you ask Dan a few questions before making some theories about whatâs causing his symptoms. You discover he just got back from Haiti where he visited friends for 2 weeks. His doctor had given him enough doxycycline for the whole trip and he had taken it religiously. His vaccination history included immunizations to typhoid, hepatitis B and tetanus. He also took Bumex. His major complaints were joint pain, nausea, and the growing rash on his trunk. His fever was 101.8. But as you were talking with him you noticed his speech began to slur a bit. This was deeply concerning.
1. Danâs speech became even more slurred and he could no longer move his arms, flaccid paralysis. You ordered an emergency CT scan that revealed encephalitis. Finally the molecular results came back. Has your tentative diagnosis been confirmed? Interpret the test and describe how it works.
Real time reverse transcriptase PCR (rRT-PCR)
The probe Cy5-ATGCAAACGGCGACCATGCCGTCAâBBQ at E1 position 10433-10456 was detected after 10 cycles
The probe JOE-AAGGACTAGMGGTTAGWGGAGACCCâBBQ at 3âUTR position 10610-10634 was not detected after 300 cycles
The probe FAM-CTYAGACCAGCTGAAR-BBQ at NS5 position 9304-9320 was not detected after 300 cycles.
Microbiology Homework Help !!!
Dan was feeling terrible! He knew he should have worn more sunscreen on that trip to Haiti. Sitting on the plane waiting to be allowed off was torture! He was burning up and felt exhausted. And sitting still so long on the plane had made his ankles and shoulders start to ache too. If only they would let him off the plane! He was beginning to feel nauseous and hoped he didnât vomit all over his fellow passengers.
After they finally let him off the plane he rushed to the bathroom to put some cool water on his face and inspect this horrible sunburn. But it didnât look normal in the mirrorâ¦. His skin was covered in red blotchy spots, especially his chest and all the way up his neck. Maybe he was allergic to something? Can allergies cause this heat (he assumed now it was a fever)? He had to go to the doctor, now. LA General was a short cab ride away.
As the PA on duty you ask Dan a few questions before making some theories about whatâs causing his symptoms. You discover he just got back from Haiti where he visited friends for 2 weeks. His doctor had given him enough doxycycline for the whole trip and he had taken it religiously. His vaccination history included immunizations to typhoid, hepatitis B and tetanus. He also took Bumex. His major complaints were joint pain, nausea, and the growing rash on his trunk. His fever was 101.8. But as you were talking with him you noticed his speech began to slur a bit. This was deeply concerning.
1. Danâs speech became even more slurred and he could no longer move his arms, flaccid paralysis. You ordered an emergency CT scan that revealed encephalitis. Finally the molecular results came back. Has your tentative diagnosis been confirmed? Interpret the test and describe how it works.
Real time reverse transcriptase PCR (rRT-PCR)
The probe Cy5-ATGCAAACGGCGACCATGCCGTCAâBBQ at E1 position 10433-10456 was detected after 10 cycles
The probe JOE-AAGGACTAGMGGTTAGWGGAGACCCâBBQ at 3âUTR position 10610-10634 was not detected after 300 cycles
The probe FAM-CTYAGACCAGCTGAAR-BBQ at NS5 position 9304-9320 was not detected after 300 cycles.