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Why is the glucose concentration in this medium so much lower than the concentration of sucrose or lactose? Triple-Sugar Iron Agar lap report #41. Microbiology lab. Organism 1.salmonella typhinurium 2. Escherichia coli 3. Shigella dysenteriae 4. Alcaligenes faecalis

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Nestor Rutherford
Nestor RutherfordLv2
28 Sep 2019

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Related questions

1. The pH for the water in the MSA recipe is expected to be from 7.2-7.6. If the pH was too low, what could you expect to see? Select one:

a. The entire plate would be yellow

b. Nothing abnormal

c. The agar would crack at pH 6.5

d. E. coli would turn purple

2. Which of the following would the MSA medium NOT be considered: Select one:

a. Complex medium

b. Chemically defined medium

c. Differential medium

d. Selective medium

3. If you took a swab from a patient and cultured it on a MSA plate, which of the following could be identified by just using this test? Select one:

a. Staphylococcus epidermidis

b. Escherichia coli

c. Staphylococcus aureus

d. Micrococcus luteus

4. If you streaked the 3 organisms in this lab on a PEA plate and after incubating for 2 days at 37 degrees Celcius nothing grew (but they did grow on a NA plate made at the same time and grown in the same conditions), which of the following is the most likely cause for this result? Select one:

a. The agar was toxic.

b. The beef extract was bad.

c. The temperature was too low.

d. Too much phenylethyl alcohol was used

. e. The temperature was too high.

5. Why is the NA plate inoculated in addition to the Columbia CNA with 5% sheep blood agar plate? Select one:

a. Used to compare growth on non-selective medium

b. Used as second selective medium

c. Used to promote hemolysis

d. Used because it is easier to see

e. Used as a practice plate

1.

If you were to make Columbia CNA with 5% sheep blood agar plates with twice as much colistin, how would the results likely be affected?

Select one:

a. Less Gram-positive growth, more Gram-negative growth

b. Less Gram-positive growth, no or even less Gram-negative growth

c. More Gram-positive growth, more Gram-negative growth

d. More Gram-positive growth, no or even less Gram-negative growth

e. Same Gram-positive growth, no or even less Gram-negative growth

2.

In Mannitol Salt Agar lab, you were told to invert the plate before incubation. This is a common practice to avoid:

Select one:

a. Condensation drops

b. Increased smell

c. Decrease oxygen content

d. Bacteria from growing in wrong direction

e. Airborne contamination

3.

If you see a yellow halo surrounding a colony on a MSA plate, which of the following is likely to be true?

Select one:

a. Glycolysis occurred

b. The organism is Staphylcoccus epidermidis

c. Colonies are not isolated

d. Fermentation occurred

e. Colony is Gram-negative

4.

Which one of the following is found in MSA plates, but not in NA plates?

Select one:

a. Phenol red

b. Agar

c. Tryptose

d. Peptone

e. Beef extract

5.

Why are the three organisms of Phenylethyl Alcohol Agar lab inoculated on the same plate?

Select one:

a. To avoid contamination

b. For easy viewing purposes.

c. To save resources

d. To avoid fluctuations in medium content and environmental effects.

6.

For which reason would you most likely use a PEA plate for culturing after taking a sample from a patient?

Select one:

a. To isolate Streptococcus and Staphylococcus from other fecal bacteria.

b. To detect Escherichia contamination on the skin.

c. To find E. coli strains associated with food poisoning.

d. To identify gram-negative anaerobes in the patient's flora.

1) Why are there few bacteria in the stomach and small intestine, but a lot in the large intestine?

2) Explain how we get enteric diseases when there are so many enterics already in our bodies.

3) Why do scientists want to test the presence of enterococci? What infections do they cause?

4) Is MacConkey Agar a selective medium or a differential medium? How do you know?

5)The lab manual states that the microbes in the large intestine and commensals and mutulastic with their host. Explain this statement with examples (give more information that just writing down the definition of commensalism and mutualism).

6) tests to determine the presence of Bacreriodes in clams and oysters are being developed. If these bacteria do not present a health hazard, what are these tests being developed?


7) You are called to investigate an outbreak of diarrheal in a child-care center. The symptoms include vomiting, fever, nausea, and cramps. You culture fecal samples from children and find a gram- negative, lactose negative rod. The bacterium does not produce gas from glucose and makes colorless colonies on MacConkey agar. Identify the genus of this bacterium.

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