1) Multiple answer. Restriction endonucleases have a number ofcharacteristics, including:
a) They were primarily isolated from phage DNA, such asbacteriophage lambda.
b) They can cut palindromic DNA sequences.
c) They are used to digest a PCR product and a plasmid and withthe aid of ligase, can be used to generate recombinant DNA such asfor protein expression.
d) They hydrolyze recombinant DNA at the ribose base.
e) They have been primarily isolated from bacteria
2) True orfalse:In quantitative PCR(qPCR) the florescence is monitored in the course of a PCRamplification, do determine the CT, the cycle at which the signalexceeds a defined threshold, and is used for example to determinethe levels of mRNA in a cell.
3) True or false: DNA microarrays use the Sanger dideoxysequencing method to help monitor changes in gene expression, suchas analyses yeast gene expression under a range of conditions suchas heat shock, nitrogen depletion and amino acid starvation.
4) Multiple answer. When conducting PCR studies, which of thefollow may occur.
a) The PCR products are isolated using a polyacrylamide gel
b) RNA-based primers are designed an used to start thepolymerase reaction
c) After 30 cycles the original piece of DNA can be amplifiedabout a billion fold.
d) The PCR products are isolated using an agarose gel
e) The PCR method can be used in forensic science to determineif a sample, such as blood is from a certain individual.
1) Multiple answer. Restriction endonucleases have a number ofcharacteristics, including:
a) They were primarily isolated from phage DNA, such asbacteriophage lambda.
b) They can cut palindromic DNA sequences.
c) They are used to digest a PCR product and a plasmid and withthe aid of ligase, can be used to generate recombinant DNA such asfor protein expression.
d) They hydrolyze recombinant DNA at the ribose base.
e) They have been primarily isolated from bacteria
2) True orfalse:In quantitative PCR(qPCR) the florescence is monitored in the course of a PCRamplification, do determine the CT, the cycle at which the signalexceeds a defined threshold, and is used for example to determinethe levels of mRNA in a cell.
3) True or false: DNA microarrays use the Sanger dideoxysequencing method to help monitor changes in gene expression, suchas analyses yeast gene expression under a range of conditions suchas heat shock, nitrogen depletion and amino acid starvation.
4) Multiple answer. When conducting PCR studies, which of thefollow may occur.
a) The PCR products are isolated using a polyacrylamide gel
b) RNA-based primers are designed an used to start thepolymerase reaction
c) After 30 cycles the original piece of DNA can be amplifiedabout a billion fold.
d) The PCR products are isolated using an agarose gel
e) The PCR method can be used in forensic science to determineif a sample, such as blood is from a certain individual.
