1. This question about FRAP will require some thinking and you maywant to draw a concept map. You genetically engineer your membraneprotein so that GPF is fused to itsC-terminus. This construct can get to the membrane on itsown and give fluorescence. You also make a second version of thesame protein, by fusing RFP to theN-terminus of the protein (RFP = red fluorescent protein).This protein can also get o to the membrane on its own and givefluorescence. Now you transfect one group of cells with theGFP-tagged protein, and a different group of cells with theRFP-tagged protein and you conduct FRAP experiments. You obtain thefollowing two FRAP graphs:
a. Label the X and Y axes for each graph and put units and put somearbitrary but reasonable numbers.
b. What is happening at the arrow?
c. Explain what is the difference between the two constructs (i.e.,what is the interpretation of the graphs). 1 sentence.
d. Give the most likelybiological explanation for this difference.
e. Propose an experimentto test this explanation.
1. This question about FRAP will require some thinking and you maywant to draw a concept map. You genetically engineer your membraneprotein so that GPF is fused to itsC-terminus. This construct can get to the membrane on itsown and give fluorescence. You also make a second version of thesame protein, by fusing RFP to theN-terminus of the protein (RFP = red fluorescent protein).This protein can also get o to the membrane on its own and givefluorescence. Now you transfect one group of cells with theGFP-tagged protein, and a different group of cells with theRFP-tagged protein and you conduct FRAP experiments. You obtain thefollowing two FRAP graphs:
a. Label the X and Y axes for each graph and put units and put somearbitrary but reasonable numbers.
b. What is happening at the arrow?
c. Explain what is the difference between the two constructs (i.e.,what is the interpretation of the graphs). 1 sentence.
d. Give the most likelybiological explanation for this difference.
e. Propose an experimentto test this explanation.
