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Describe in detail how you would:


1.Make four different concentrations of a solution of knownconcentration (for example, 400 mM Orange G).

2.Use Beer's Law to calculate the concentrationof an unknown dilution of a solution (in this example, OrangeG).

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Reid Wolff
Reid WolffLv2
28 Sep 2019

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Please answer all the questions answering only one question is not helpful :

Calculations on Multiple Stock Solutions—be sure to include the amount of water added and include all units

11. Calculate how to use the 1 M Tris and 0.2 M EDTA stock solutions to make the 400 ml of a of 10 mM Tris and 1 mM EDTA solution.

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17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.

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P2 P10 P20 P100 P200 P1000

22. How accurate was your pipetting?

23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.

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