Based upon your results, is your bacteria sample a strictaerobe, strict anaerobe, facultative anaerobe, or microaerophile?Why? â Look at your results from OF Glucose and OF Glucose with oiltest.
How important is it to add the Kovacâs Reagent in the IndoleUtilization test? What is responsible for the color change observedin a positive test? âYou can read more about this test in the TestMedia Reference in VUMIE2012. Click the M? button.
Kovacâs reagent consists ofp-dimethylaminobenzaldehyde dissolved in HCl. The reagent is addedto an overnight culture of the unknown in tryptone broth.Development of a red ring atop the broth is indicative of theproduction of indole, a breakdown product in the digestion of theamino acid tryptophan.
Did the bacteria grow in the tryptone or malonate media? Why orwhy not? â Remember, you can tell if they grew if culture wasturbid (cloudy).
For the media used in this portion of the experiment, are themedia complex or synthetic? Are the media selective, differential,both, or neither? Identify the carbon and nitrogen source for eachmedium. â Read more about what these terms mean starting on page178 in your book right below Figure 6.11. Look up each media withthe M? button. Remember, nutrient broth is a complex media, mineralsalts is a defined media.
How many possible bacteria were remaining after conducting thesebiochemical tests?
Using the information in the Identification matrix, whatadditional tests might help you to identify the bacteria in yourcase study? âLook at the View Identification Matrix under the Viewmenu. What tests would you perform? Look for ones that have a mixof + and â because they would help distinguish betweenorganisms.
Based upon your results, is your bacteria sample a strictaerobe, strict anaerobe, facultative anaerobe, or microaerophile?Why? â Look at your results from OF Glucose and OF Glucose with oiltest.
How important is it to add the Kovacâs Reagent in the IndoleUtilization test? What is responsible for the color change observedin a positive test? âYou can read more about this test in the TestMedia Reference in VUMIE2012. Click the M? button.
Kovacâs reagent consists ofp-dimethylaminobenzaldehyde dissolved in HCl. The reagent is addedto an overnight culture of the unknown in tryptone broth.Development of a red ring atop the broth is indicative of theproduction of indole, a breakdown product in the digestion of theamino acid tryptophan.
Did the bacteria grow in the tryptone or malonate media? Why orwhy not? â Remember, you can tell if they grew if culture wasturbid (cloudy).
For the media used in this portion of the experiment, are themedia complex or synthetic? Are the media selective, differential,both, or neither? Identify the carbon and nitrogen source for eachmedium. â Read more about what these terms mean starting on page178 in your book right below Figure 6.11. Look up each media withthe M? button. Remember, nutrient broth is a complex media, mineralsalts is a defined media.
How many possible bacteria were remaining after conducting thesebiochemical tests?
Using the information in the Identification matrix, whatadditional tests might help you to identify the bacteria in yourcase study? âLook at the View Identification Matrix under the Viewmenu. What tests would you perform? Look for ones that have a mixof + and â because they would help distinguish betweenorganisms.
