Microbial Growth
You are designing an experiment to understand the effect thattemperature has on the
growth of your bacterium, Enterococcus faecium. You are tryingto answer the following
questions.
a) What is the population number at the end of your experimentif the bacterium is grown at 37oC?
b) Will the population number be affected if the temperature isreduced from 37oC to 25oC? If so, how?
a. e.g. Do you expect to population size to increase, decreaseor remain the
same?
b. Provide a rationale for your answer.
You know the following pieces of information.
a) Doubling time for your bacterium is 20 minutes at 37oC.
b) You will allow your culture to grow exponentially for 8hours.
c) The initial number of cells used to start your experiment is5 x 10^3
.
d) When grown at 37oC for 12 hours, there are 5 x10^8
cells at the end of the experiment.
e) Enterococci are able to grow at different ranges oftemperature (5oC-65oC) and pH
(4.5-10), but 42.7 is optimum.
Microbial Growth
You are designing an experiment to understand the effect thattemperature has on the
growth of your bacterium, Enterococcus faecium. You are tryingto answer the following
questions.
a) What is the population number at the end of your experimentif the bacterium is grown at 37oC?
b) Will the population number be affected if the temperature isreduced from 37oC to 25oC? If so, how?
a. e.g. Do you expect to population size to increase, decreaseor remain the
same?
b. Provide a rationale for your answer.
You know the following pieces of information.
a) Doubling time for your bacterium is 20 minutes at 37oC.
b) You will allow your culture to grow exponentially for 8hours.
c) The initial number of cells used to start your experiment is5 x 10^3
.
d) When grown at 37oC for 12 hours, there are 5 x10^8
cells at the end of the experiment.
e) Enterococci are able to grow at different ranges oftemperature (5oC-65oC) and pH
(4.5-10), but 42.7 is optimum.
