Question

Your assistant Igor is studying a neurotransmitter-stimulatedGPCR pathway, hypothesized to stimulate the expression of genesthat increase retention of memory. He plans to test his hypothesisusing a neural cell line expressing this GPCR. If his hypothesis iscorrect, what would be the expected outcome on memory geneexpression? Explain your reasoning for each answer.

a. Stimulation of cAMP phosphodiesterase

b. Treatment of cells with cholera toxin

c. Increased affinity of PKA for cAMP

d. a mutation in the nuclear import sequence of PKA fromPPKKKRKV to PPAAAAAV

e. deletion of a functional phosphatase for the gene regulatoryprotein