The Lac repressor protein binds to the:

A)
lacZ gene in the presence of allolactose.

B)
operator in the presence of allolactose.

C)
operator in the absence of allolactose.

D)
operator regardless of the presence or absence ofallolactose.

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jyannLv7

2 Mar 2023

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Lelia LubowitzLv2

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1. The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/).

The possible answers indicate with a Êº+Êº or a Êº-Êº whether Î²-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)

Genotype A: I + O+ Z+/ FÊ¹ I - O+ Z+

KEY:

I+ = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)

a. +,+ d. -,+

b. -,- e. cannot tell for certain

c. +,-

2. The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/).

KEY:

I+ = wild-type repressor

I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)

a. +,+ d. -,+

b. -,- e. cannot tell for certain

c. +,-