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28 Sep 2019
A cloned DNA sequence was used to probe a Southern blot. Therewere 2 DNA samples on the blot, one from white blood cells and theother from a liver biopsy of the same individual. Both samples hadbeen digested with HpaII. The probe bound to a single 2.2 kb bandin the white blood cell DNA, but bound to 2 bands (1.5 and 0.7 kb)in the liver DNA.
a ) What are possible explanations for this difference?
b ) How would it affect your answer if you knew that white bloodcell and liver DNA from this individualboth showed the 2 bandpattern when digested with MspI ?
A cloned DNA sequence was used to probe a Southern blot. Therewere 2 DNA samples on the blot, one from white blood cells and theother from a liver biopsy of the same individual. Both samples hadbeen digested with HpaII. The probe bound to a single 2.2 kb bandin the white blood cell DNA, but bound to 2 bands (1.5 and 0.7 kb)in the liver DNA.
a ) What are possible explanations for this difference?
b ) How would it affect your answer if you knew that white bloodcell and liver DNA from this individualboth showed the 2 bandpattern when digested with MspI ?
Elin HesselLv2
28 Sep 2019
