Question

5). The table shows five different mutations of a small section of a gene.

Use the base-pairing rules to complete the second column. For each mutation, write the sequence for any mRNA codons that will be changed as a result of the mutation in bold. Write any codons that won’t be changed in regular font.

Using the genetic code chart from question #3, identify any stop codons in the mRNA molecules – write these codons in red

For each mutation, write any amino acids that will be changed in the last column in bold font. Use red XXXs to indicate any amino acids that will be missing as a result of stop codons and use use regular font indicate any amino acids that will not be changed.

DNA (Template Strand)	mRNA codon	Polypeptide
Original DNA = GCAAGTACCTGA	CGU UCA UGG ACU	arg – ser – trp – thr
Mutation = GCCAGTACCTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCACGTACCTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCAAGTACTTGA (nucleotide change underlined)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GAAGTACCTGA (first C deleted)	____ ____ ____ ____	___ ___ ___ ___
Mutation = GCAAGTACTGA (second C deleted)	____ ____ ____ ____	___ ___ ___ ___