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In which direction (into the cell or out of the cell) will waterflow in cells of Escherichia coli suddenly suspended in a solutionof 20% NaCl? What if the cells were suspended in distilled water?If growth nutrients were added to each suspension, which (ifeither) would support growth and why? How would you classify E.coli in terms of its salt preferences? Explain.

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Trinidad Tremblay
Trinidad TremblayLv2
28 Sep 2019

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Related questions

Directions: Write out your answers in the word document and then copy and paste everything into the text box. If you do not include the question along with your answer, you will be marked down.

1. What would happen if you put pGLO directly onto an agar plate and you checked on it after letting it incubate?

a. It would glow

b. It would grow

c. Nothing

d. E.Coli would grow

2. Explain in a short sentence or two your reasoning for question 1.

3. What shape is a plasmid?

4. How is a plasmid different from human DNA? Write one difference.

5. Bacteria can share DNA through a unique method called transformation. What are the TWO requirements a bacteria cell must possess in order to share DNA with another bacteria cell?

6. Why during our experiment did we put our e.Coli with pGLO inserted in it onto agar plates with ampicillin (Amp)?

a. So that it would kill the e.Coli and serve as our control

b. To kill all the e.Coli that did not take up the pGLO

c. To create antibiotic resistant bacteria

d. To cause the e.Coli to glow

7. Yeast cells are what type of cell?

a. Bacteria

b. Eukaryotes

c. Prokaryotes

d. Archaea

8. Why would someone study yeast cells instead of human cells?

9. Do you agree or disagree with the following statement: If your cells is exposed to UV light and it causes a thymidine dimer, your cell is doomed to die.

10. Are wildtype (control) yeast immune to UV light damage?

11. BTB was a dye we used to directly measure ________

a. PH

b. Oxygen

c. Carbon Dioxide

d. Carbon

12. BTB was a dye we used to INDIRECTLY measure ________

a. PH

b. Oxygen

c. Carbon Dioxide

d. Carbon

13. What is one reason why a plant would want to close its stomata?

14. What is one function that ground tissues provides for a plant?

15. List one difference between monocots and dicots

16. Why is it important for a plant to have stomata? Write one reason why

17. What is the purpose of xylem in a plant cell?

18. Pholem is also important to plant cells, what is it’s purpose?

19. This theory explains why trees can grow to such tall heights and still provide water to their leaves.

a. Translocation

b. Transpiration

c. Cohesion-Tension

d. Vacuum

20. Fill in the blank

Pholem is considered to be a(n) _________ transport system while xylem is considered a(n) _______________transport system.

21. Apical meristems are found in these two places on a plant

a. At the site of new leaf growth and the roots closes to the top of the soil

b. Vascular tissue and ground tissue

c. Ground tissue and dermal tissue

d. The point of new growth on the stem and root

22. Fill in the blank.

Xylem moves substances _____ while phloem moves substances _____ a plant.

23. What are the two types of digestive mechanisms?

24. What is one benefit that mastication has for digestion?

25. What is an example of an organism that digests food intracellularly?

26. What is an example of an organism that digests food extracellularly?

27. Give two examples of where surface area is increased in a small space to aid in absorption within the human body

28. Name one difference between arteries and veins.

29. What is the role of capillaries in the circulatory system?

30. How are the terms “excretion” and “defecation” different?

31. What is the role of the ADH hormone?

32. What does the term “Medulla” refer to when talking about mammalian tissue?

a. The outer edges of a tissue

b. The middle of a tissue

c. The top of a tissue

d. The bottom of a tissue

33. Fill in the blank

Mammals have a _______ circulatory system while insects have a _______ circulatory system.

34. What is one benefit a closed circulatory system provides?

35. What is hemolymph?

1. In lecture, we described a simple osmotic pressure gradient when discussing osmosis. For question #1, describe the simple osmotic pressure gradient, in terms of osmotic pressure, that exists across the red blood cell plasma membrane when red blood cells are suspended in a severely hypotonic solution, such as a 0.2% NaCl solution. Use scientific terminology.

2. We mentioned in class that biological systems are always in a state of osmotic equilibrium due to a combination of solute diffusion in some cases and osmosis in others. In this experiment, when you add the red blood cells to a severely hypotonic solution, what substances are moving across the red blood cell plasma membrane to achieve osmotic equilibrium, Na+ and Cl- or water? Explain why based on your understanding of the permeability characteristics of biological membrane. Always use scientific language.

CAN QUESTIONS 1 AND 2 BE ANSWERED DIRECTLY AND ADEQUATLEY PLEASE.

HERE IS AN INTRO TO THE LAB EXPERIMENT

Intro:

We have known for a very long time that the mammalian red blood cell plasma membrane is very permeable to water. Even the introductory biology student understands that if you put mammalian red blood cells in water, they will rapidly swell and break open (hemolyze) due to the rapid osmosis of water into the cell. The experimental question we are asking in today's lab experiment is "Is the high water permability of mammalian red blood cell plasma membrane due solely to the lipid bilayer, or are there water channels present in the membrane?"

Experimental Design:

It has been shown by several investigators that water movement through water channels can be blocked by the known K+ channel blocker, tetraethylammonium chloride (TEAC). Brooks, Regan and Yool (2000) showed that TEAC reduces the water permeability of human water channels expressed in the plasma membranes of frog ocytes. After preincubation of the oocytes for 15 min with 100 microM TEAC, the water permeability of frog oocyte plasma membrane containing human water channels was reduced 20 to 40%. The investigators concluded that treatment of cells with TEAC can be useful in determining the presence of aquaporins in membranes.The specific objective of today's experiment is to uncover the presence of water channels in mammalian red blood cell plasma membrane by determining the effect of tetraethylammonium chloride on the water permeability of mammalian RBC plasma membrane.

Post-Lab Questions

For each of the tubing pieces, identify whether the solution inside was hypotonic, hypertonic, or isotonic in comparison to the beaker solution in which it was placed.

Which tubing increased the most in volume? Explain why this happened.

What do the results of this experiment this tell you about the relative tonicity between the contents of the tubing and the solution in the beaker?

What would happen if the tubing with the yellow band was placed in a beaker of distilled water?

How are excess salts that accumulate in cells transferred to the blood stream so they can be removed from the body? Be sure to explain how this process works in terms of tonicity.

If you wanted water to flow out of a tubing piece filled with a 50% solution, what would the minimum concentration of the beaker solution need to be? Explain your answer using scientific evidence.

How is this experiment similar to the way a cell membrane works in the body? How is it different? Be specific with your response.

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