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Microbiology : List and describe the function of the 4 essential genetic regions of the pGLO plasmid used in the bacterial transformation experiment and describe what they do .

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Nelly Stracke
Nelly StrackeLv2
28 Sep 2019

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Related questions

1. Carefully consider the options and match the conditions (ofplasmid and plate) with the expected growth.

+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid

-pGLO on LB/Amp?

-pGLO on LB/Ara?

+pGLO on LB/Ara?

Possible answers include:

a. No growth at all

b. Growth of both white color and green fluorescent colonies

c. Growth of only green fluorescent color colonies

d. Growth of only white color colonies

2. What genes are required in the pGLO plasmid for production offluorescent bacteria on LB/Ara plates?

There may be more than one correct answer. Identify all forcredit.

a. None of these genes

b. bla

c. araC

d. All of these genes

e. GFP

3. What genes are required in the pGLO plasmid for growth ofe. coli bacteria on nutrient broth plates?

There may be more than one correct answer. Identify all forcredit.

a. None of these genes

b. bla

c. araC

d. All of these genes

e. GFP

(Side note to help answer question: The transformationexperiment we did we used pGLO (5400 bp) so this question below isasking what would happen if we repeated the experiment but used a15 kb plasmid)

1. Assume that you repeat this transformation using a 15 kbplasmid. Would this affect the outcome of the experiment? Give aDETAILED explanation!

GIVEN = In a bacterial transformation lab ( you are trying to transform e.coli to glow under UV light), you do 4 plates.

Transformation plates = +pGLO LB/amp and +pGLO LB/amp/ara

Control Plates = -pGLO LB/amp and -pGLO LB

(a) What 2 factors must be present in the bacteria's environment for you to see the green color?

(b) EXPLAIN IN DETAIL how the 2 environmental factors form part (a) causes genetically transformed bacteria to glow green?

(c) What advantages would there be for an organism to be able to turn on or off particular genes in response to certain conditions? EXPLAIN

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